2P265 Super-resolution imaging of chromatin domains in living mammalian cells(21B. Genome biology:Genome structure,Poster)

Genomic DNA must be organized three dimensionally in the cell to utilize genome information. Our recent studies demonstrated the nucleosome fiber is irregularly folded in the cell without the 30-nm chromatin fiber. Using newly developed single nucleosome imaging, we identified numerous compact nucleosome clusters (chromatin domains) in both interphase chromatin and mitotic chromosomes in living mammalian cells. Subsequently, we also observed that nucleosomes dynamically moved around in the chromatin domains. Furthermore, we detected that some chemical perturbations could change the chromatin domains and nucleosome dynamics. These findings provide a novel insight into the genome organization and dynamics in living mammalian cells.