极端环境微生物“IISA-Gene”基因原位集成分析仪的研制与评价

T. Fukuba, T. Fujii
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引用次数: 1

摘要

本研究开发了一种基因分析集成原位分析系统(IISA-Gene),用于对深海或深层地下等极端环境下的微生物进行基因分析。采用PDMS和玻璃制成的微晶片,实现了系统的完全小型化和集成化。IISA-Gene系统包括微型泵浦和光学检测装置,可进行细菌细胞裂解、DNA纯化、PCR扩增和扩增片段检测。本研究开发的iisa基因在真实的深海环境中进行了评估和操作
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development and Evaluation of the Integrated In Situ Analyzer for Gene-"IISA-Gene" for Microbiology in Extreme Environments
In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment
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