{"title":"基于分子标记的Sciaenae双雄蕊蝇(单属，微子叶科)重描述、新寄主记录及其系统发育状况","authors":"A. Verma, Jyoti Verma, Nirupama Agrawal","doi":"10.2478/vzoo-2018-0005","DOIUrl":null,"url":null,"abstract":"Abstract A new host and new locality is recorded for Diplostamenides sciaenae (Goto, 1894) Lebedev, Parukhin et Roitman, 1970 from Johnius belangerii at Versova dock landing centre, Mumbai, India. The morphometric comparison of D. sciaenae with previously published data, provided redescription complements results of molecular analysis. The partial 28S and 18S rRNA gene sequences of D. sciaenae were amplified, sequenced through PCR and deposited to GenBank database. The BLASTn searches revealed the significant closeness of D. sciaenae to other microcotylid parasites in large and small ribosomal subunits. Th e phylogenetic tree analyses with neighbor joining and minimum evolution methods also expressed belonging of D. sciaenae to Microcotylidae.","PeriodicalId":206426,"journal":{"name":"Vestnik Zoologii","volume":"37 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Redescription and New Host Record of Diplostamenides Sciaenae (Monogenea, Microcotylidae) and Its Phylogenetic Status Using Molecular Markers\",\"authors\":\"A. Verma, Jyoti Verma, Nirupama Agrawal\",\"doi\":\"10.2478/vzoo-2018-0005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract A new host and new locality is recorded for Diplostamenides sciaenae (Goto, 1894) Lebedev, Parukhin et Roitman, 1970 from Johnius belangerii at Versova dock landing centre, Mumbai, India. The morphometric comparison of D. sciaenae with previously published data, provided redescription complements results of molecular analysis. The partial 28S and 18S rRNA gene sequences of D. sciaenae were amplified, sequenced through PCR and deposited to GenBank database. The BLASTn searches revealed the significant closeness of D. sciaenae to other microcotylid parasites in large and small ribosomal subunits. Th e phylogenetic tree analyses with neighbor joining and minimum evolution methods also expressed belonging of D. sciaenae to Microcotylidae.\",\"PeriodicalId\":206426,\"journal\":{\"name\":\"Vestnik Zoologii\",\"volume\":\"37 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik Zoologii\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/vzoo-2018-0005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Zoologii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/vzoo-2018-0005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
Lebedev, Parukhin et Roitman, 1970年在印度孟买Versova船坞登陆中心的Johnius belangerii记录了一种新的寄主和新的地点。与先前发表的数据进行了形态计量学比较，为分子分析结果提供了补充。扩增sciaenae部分28S和18S rRNA基因序列，通过PCR测序并存入GenBank数据库。BLASTn搜索结果显示，sciaenae与其他微子叶寄生虫在大小核糖体亚基上具有显著的相似性。采用邻居连接法和最小进化法进行的系统发育树分析也表明了该物种属于微子叶科。
Redescription and New Host Record of Diplostamenides Sciaenae (Monogenea, Microcotylidae) and Its Phylogenetic Status Using Molecular Markers
Abstract A new host and new locality is recorded for Diplostamenides sciaenae (Goto, 1894) Lebedev, Parukhin et Roitman, 1970 from Johnius belangerii at Versova dock landing centre, Mumbai, India. The morphometric comparison of D. sciaenae with previously published data, provided redescription complements results of molecular analysis. The partial 28S and 18S rRNA gene sequences of D. sciaenae were amplified, sequenced through PCR and deposited to GenBank database. The BLASTn searches revealed the significant closeness of D. sciaenae to other microcotylid parasites in large and small ribosomal subunits. Th e phylogenetic tree analyses with neighbor joining and minimum evolution methods also expressed belonging of D. sciaenae to Microcotylidae.
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