Observational Survey to Assess Platelet Concentration and Ani - Microbial Potency of Different Blood Extracts

Introduction: Platelet concentrates have gained popularity in periodontal regenerative therapy because of its autologous nature. it promotes wound healing after surgical periodontal therapy. Aim: to study the platelet count and antimicrobial efficacy of various platelet concentrates, such as PRP, PRF and IPRF Methodology: An Observational Study was carried out at Department of Periodontology & Implantology at Babu Banarasi das College of Dental Sciences. Sample size was 45. Ten ml of blood was drawn from the patient out of which 3ml each of blood will be used for PRP, PRF and I-PRF preparation and remaining 1ml of blood was used for determining the platelet count. For I-PRF preparation, 3ml of blood wias used, d centrifuged at 700 rpm for 3 minutes. For PRF preparation, Agar plates will be inoculated with plaque sample of same patient and will be labelled and divided into 3 compartments. Wells was prepared in the inoculated agar plate and 0.1 ml of PRP, PRF and I-PRF will be placed in those wells. Inoculated blood agar plates was then incubated aerobically at 37℃ for 24 hours to 48 hours. Results: The platelet count of i-PRF was statistically significant when compared to control (P < 0.001). It was also significant when compared to PRP (P < 0.01) and PRF (P < 0.001). Mean zone of inhibition around i-PRF (P < 0.01) and PRF (P < 0.05) reached statistical significance. Although a distinct zone of inhibition was seen with PRP, it was not statistically significant (P > 0.05). Conclusion: In the present study, although a distinct zone of inhibition was obtained with all test samples, it was significant with only i-PRF and PRF.