体外外周血单核和多形核细胞对恶性疟原虫生长和白细胞介素-8产生的抑制作用。

Julieta Yuri Ohya, H. Matsuoka, N. Mukaida, T. Kasahara, A. Ishii
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引用次数: 0

摘要

外周血单核细胞和多形核细胞已被证明能在体外杀死和抑制疟原虫的增殖,但大多数报道需要细胞因子或免疫血清或调节素的存在来激活细胞。在我们的研究中,来自非免疫供体的外周血白细胞有效地抑制恶性疟原虫的生长,这取决于效应器/靶比。此外,这些细胞在感染的红细胞或恶性疟原虫培养物上清的刺激下产生大量的白细胞介素-8 (IL-8)。多形核细胞的培养上清液中IL-8的分泌在寄生虫刺激6-9小时时被观察到,在培养24小时后显著增加。我们试图阐明IL-8是否参与疟疾抑制,结果表明IL-8不直接参与单核和多形核细胞的抗疟疾活性。除IL-8外,其他抑制疟原虫生长的机制可能与白细胞相关的疟原虫培养系统有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Growth inhibition of Plasmodium falciparum and interleukin-8 production by peripheral blood mononuclear and polymorphonuclear cells in vitro.
Peripheral blood mononuclear and polymorphonuclear cells have been demonstrated to kill and inhibit malaria parasite proliferation in vitro, but most of the reports required activation of the cells by cytokines or presence of immune sera or opsonins. In our study, peripheral blood leukocytes from non-immune donor efficiently inhibited Plasmodium falciparum growth, depending on the effecter/target ratio. Moreover, these cells produced a large amount of interlenkin-8 (IL-8) under stimulation with infected erythrocytes or supernatant of the Plasmodium falciparum culture. IL-8 secretion in the culture supernatant of polymorphonuclear cells was noted from 6-9 hr of stimulation with the parasites, with a substantial increase over 24 hours of culture. Attempt to elucidate whether IL-8 was involved in malaria suppression was done, and the result suggested that IL-8 was not directly involved in the anti-malarial activity of the mononuclear and polymorphonuclear cells. The other mechanism besides IL-8 may work for inhibiting parasite growth in the culture system of malaria parasites associated with leukocytes.
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