Intan Shafinaz Mohamad Jamal, Nurul Syuhada Sahari, Siti Halimah Hasmoni, Z. Ibrahim, N. Malek, S. Shahir
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引用次数: 0
摘要
本研究旨在探索从木薯废水中分离生物纤维素(BNC)产菌的潜力。在Hestrin and Schramm (HS)培养基中成功分离出10株细菌,其中只有1株BPNC 3产生白色凝胶状物质,被认为是BNC。根据16S rRNA分析,BPNC3为Asaia sp.菌株。BPNC3通过傅里叶红外光谱(FTIR)和场发射扫描电镜(FESEM)对菌株进行了表征。FTIR光谱显示,在3276.69 cm-1和2923.99 cm-1处存在两个特征峰,表明纳米纤维素材料。FESEM显微照片显示了纳米纤维素结构中典型的网状纤维特征。
Characterization of Bionanocellulose Producing Bacteria Isolated from Tapioca Wastewater
This study was conducted to explore the potential of isolating bionanocellulose (BNC) producing bacteria from tapioca wastewater. A total of ten bacteria were successfully isolated and only one isolate named BPNC 3 produced white gelatinous materials in Hestrin and Schramm (HS) medium believed to be the BNC. According to 16S rRNA analysis, bacterium BPNC 3 was identified as Asaia sp. The BNC produced by Asaia sp. BPNC3 was characterized via Fourier transformed-infrared spectroscopy (FTIR) and Field-emission scanning electron microscope (FESEM). The FTIR spectrum showed the presence of two signature peaks at 3276.69 cm-1 and 2923.99 cm-1 indicative of nanocellulosic material. The FESEM micrograph showed characteristics of network fibrils typically present in nanocellulose structure.