发育生物学中细胞纺锤体的双光子激发荧光成像

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI:10.1117/12.741582

Liang Gao, Wenjia Bai, Lei Jin, P. Xue, Hui Ma, Dieyan Chen

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引用次数: 0

摘要

将双光子激发荧光(TPEF)技术集成到共聚焦光学系统中，首次获得了高分辨率的ICR(内控区)大鼠受精卵纺锤体的TPEF图像。实验结果表明，TPEF是研究胚胎早期发育阶段细胞纺锤体的有效探针。

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Two-photon excited fluorescence imaging of cell spindles for developmental biology

By integrating two-photon excited fluorescence (TPEF) technique into confocal optical system, TPEF images of zygote spindles of ICR (Internal Control Region) rats have been obtained in high resolution for the first time. Experimental results demonstrate the validity of TPEF as an efficient probe in investigating cell spindles at early embryonic developmental stage.

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International Conference on Photonics and Imaging in Biology and Medicine

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