原卟啉(PPIX)与5-氨基乙酰丙酸(ALA)在纳米乳中的相互作用

M. Iwunze
{"title":"原卟啉(PPIX)与5-氨基乙酰丙酸(ALA)在纳米乳中的相互作用","authors":"M. Iwunze","doi":"10.15406/mseij.2022.06.00188","DOIUrl":null,"url":null,"abstract":"Nanoemulsion is an interesting and unique fluid system in that it is used to solubilize both ionic and non-ionic molecules. Because of its uniqueness it has been used as a medium for drug delivery. It is therefore used in this work to study the interaction of Protoporphyrin (PPIX) and 5-Amino levulinic acid (ALA). While ALA is a distant precursor of PPIX, PPIX itself is not only a precursor of Heme but also a photosensitizer in the modality of Photodynamic Therapy (PDT). Both compounds are used use in PDT regimen. A steady-state fluorescence technique is used for the study of the interaction of these very important biological compounds. It is found that ALA quenches the fluorescence of PP IX in nanoemulsion. This observed quenching is diffusion controlled. The bimolecular quenching constant, kq, was determined as 2.86x 1010/M-s with an interaction constant, Ka, of 4.48 x 105 with the free energy of interaction, ΔGa of -32.234kJ/mole.","PeriodicalId":435904,"journal":{"name":"Material Science & Engineering International Journal","volume":"45 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interaction of protoporphyrinix (PPIX) and 5-amino levulinic acid (ALA) in nanoemulsion\",\"authors\":\"M. Iwunze\",\"doi\":\"10.15406/mseij.2022.06.00188\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Nanoemulsion is an interesting and unique fluid system in that it is used to solubilize both ionic and non-ionic molecules. Because of its uniqueness it has been used as a medium for drug delivery. It is therefore used in this work to study the interaction of Protoporphyrin (PPIX) and 5-Amino levulinic acid (ALA). While ALA is a distant precursor of PPIX, PPIX itself is not only a precursor of Heme but also a photosensitizer in the modality of Photodynamic Therapy (PDT). Both compounds are used use in PDT regimen. A steady-state fluorescence technique is used for the study of the interaction of these very important biological compounds. It is found that ALA quenches the fluorescence of PP IX in nanoemulsion. This observed quenching is diffusion controlled. The bimolecular quenching constant, kq, was determined as 2.86x 1010/M-s with an interaction constant, Ka, of 4.48 x 105 with the free energy of interaction, ΔGa of -32.234kJ/mole.\",\"PeriodicalId\":435904,\"journal\":{\"name\":\"Material Science & Engineering International Journal\",\"volume\":\"45 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Material Science & Engineering International Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15406/mseij.2022.06.00188\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Material Science & Engineering International Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/mseij.2022.06.00188","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

纳米乳液是一种独特而有趣的流体体系,它既可以溶解离子分子,也可以溶解非离子分子。由于其独特性,它已被用作药物输送的媒介。因此,在这项工作中研究了原卟啉(PPIX)和5-氨基乙酰丙酸(ALA)的相互作用。虽然ALA是PPIX的远端前体,但PPIX本身不仅是血红素的前体,而且在光动力治疗(PDT)模式中也是光敏剂。这两种化合物都用于PDT治疗方案。稳态荧光技术用于研究这些非常重要的生物化合物的相互作用。在纳米乳中发现ALA猝灭PP IX的荧光。这种观察到的淬火是扩散控制的。测定了双分子猝灭常数kq为2.86 × 1010/M-s,相互作用常数Ka为4.48 × 105,相互作用自由能ΔGa为-32.234kJ/mol。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interaction of protoporphyrinix (PPIX) and 5-amino levulinic acid (ALA) in nanoemulsion
Nanoemulsion is an interesting and unique fluid system in that it is used to solubilize both ionic and non-ionic molecules. Because of its uniqueness it has been used as a medium for drug delivery. It is therefore used in this work to study the interaction of Protoporphyrin (PPIX) and 5-Amino levulinic acid (ALA). While ALA is a distant precursor of PPIX, PPIX itself is not only a precursor of Heme but also a photosensitizer in the modality of Photodynamic Therapy (PDT). Both compounds are used use in PDT regimen. A steady-state fluorescence technique is used for the study of the interaction of these very important biological compounds. It is found that ALA quenches the fluorescence of PP IX in nanoemulsion. This observed quenching is diffusion controlled. The bimolecular quenching constant, kq, was determined as 2.86x 1010/M-s with an interaction constant, Ka, of 4.48 x 105 with the free energy of interaction, ΔGa of -32.234kJ/mole.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信