DNA与电活性配体相互作用的快速电化学探针

Proceedings of the 2006 IEEE Sensors Applications Symposium, 2006. Pub Date : 2006-06-05 DOI:10.1109/SAS.2006.1634226

C. Yang, Sufen Wang, K. Mahajan, P. Jackson, J. Schmalzel

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引用次数: 2

摘要

设计了一种基于不可逆过程电化学方程的DNA与电活性配体相互作用的快速研究方法。结合常数(K)和结合位点的大小(s)是在伏安法中由电流对添加DNA量的依赖同时得到的。实验检测了一种非插入性结合剂(Hoechst 33258)和两种dna插入剂(米托蒽醌(MXT)和放线菌素D (AMD))。结果表明，Hoechst 33258与米托蒽醌和放线菌素D的结合常数分别为2.1×10 8、8.9×10 9和9.1×10 9 cm 3 mol I 1;它们的结合位点大小分别为4、3和8。该研究提供了一种方便、灵敏的方法来估计亲和参数，并概述DNA与电活性靶向化合物之间的相互作用。

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A rapid electrochemical probe for DNA and electroactive ligand interaction

A rapid method for investigating interactions between DNA and electroactive ligands based on an electrochemical equation for irreversible processes is designed. Binding constants (K) and sizes of binding sites (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in the experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1×10 8 , 8.9×10 9 and 9.1×10 9 cm 3 mol I 1 ; and the sizes of their binding sites were 4, 3 and 8, respectively. This study provides a convenient and sensitive approach to estimating affinity parameters and outlining interactions between DNA and electroactive targeting compounds.

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Proceedings of the 2006 IEEE Sensors Applications Symposium, 2006.

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