人三倍体细胞株。细胞水平上的表型。

Humangenetik Pub Date : 1975-11-06 DOI:10.1007/BF00291945
A M Kuliev, V I Kukharenko, K N Grinberg, A T Mikhailov, A D Tamarkina
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引用次数: 4

摘要

对自然流产三倍体细胞株的生物学特性进行了复杂的研究。细胞形态学、放射自显像、细胞化学、生化和免疫化学研究表明,根据大多数研究性质,三倍体细胞与正常二倍体细胞没有区别。细胞形态正常,定向良好,在15- 17代培养中显示出表达的原纤维结构和活力。碱性磷酸酶水平降低,酸性磷酸酶、乳酸脱氢酶和丙酸脱氢酶水平升高,细胞核面积增大是与对照的本质差异。细胞有丝分裂周期参数正常,抗原谱与正常细胞基本一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human triploid cell strain. Phenotype on cellular level.

The complex investigation of the bilogical properties of the triploid cell strain derived from a spontaneous abortus was carried out. Cytomorphological, autoradiographic, cytochemical, biochemical and immunochemical investigation showed that, according to most of the investigated properties, triploid cells did not differ from normal diploid cells. The cells had normal form, were well orientated, revealed expressed fibrillar apparatus and viability in the culture during 15--17 passages. The decrease of the alkaline phosphatase level, increase of acid phosphatase, lactate and malatdehydrogenase and greater nuclei area were the essential differences from the control. The cells had normal mitotic cycle parameters and the antigenic spectrum was practically identical to the normal cells.

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