Z. Xiaohui, Ye Zhibiao, Zhang Yuyang, Hou Zheng, Li Hanxia
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Plant gene targeting and gene replacement: application to crop genetic improvement
Recent advances and limitations of using zinc finger nuclease to stimulate a high efficiency of homologous recombination, and chimeric oligonucleotides to promote single base replacement in functional genomic research and plant genetic breeding, are systematically reviewed. Approaches to improve gene targeting efficiency through molecular modification of key pathways in plant homologous recombination are also discussed.
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