番茄废提取物(Lycopersicon esculentum)在加热豆油中的天然抗氧化剂研究

I. R. Freitas, Tayane Lise Siqueira Machado, D. M. M. Luzia, N. Jorge
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引用次数: 2

摘要

番茄加工业产生大量的废物,通常由果皮和种子组成。众所周知,番茄富含生物活性化合物,主要是类胡萝卜素(番茄红素和-胡萝卜素),然而,对其残留物功能的研究很少。因此,这些残留物在不知道可能的抗氧化潜力的情况下被丢弃。本研究的目的是在加热条件下测定农业工业番茄废渣提取物中加入大豆油的生物活性的稳定性。首先用DPPH和-胡萝卜素/亚油酸分析以1:5 (w/v)乙醇提取的提取物的总酚类化合物、番茄红素、-胡萝卜素和抗氧化活性。随后,将提取液与合成的抗氧化剂丁基羟基甲苯(BHT)和叔丁基对苯二酚(TBHQ)分别应用于大豆油中,并在熏蒸炉中加热(180℃/5 h),通过高效液相色谱(HPLC)分析了热氧化样品中生育酚的组成,通过气相色谱(GC)分析了植物甾醇的组成,分析了脂肪酸的组成。农工番茄残渣提取物的抗氧化活性中等,DPPH法抗氧化率为68%,-胡萝卜素/亚油酸体系自氧化率为26.2%。与对照组相比,合成抗氧化剂TBHQ对大豆油有更大的保护作用,提供了更高的植物甾醇(85%)和生育酚(93%)的保留率。番茄提取物可以作为一种天然抗氧化剂在工业油脂中使用,但效果不如抗氧化剂TBHQ。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tomato waste extract (Lycopersicon esculentum) as a natural antioxidant in soybean oil under heating
The tomato processing industry generates a large amount of waste, usually composed of peels and seeds. It is known that the tomato is rich in bioactive compounds, mainly carotenoids (lycopene and ?-carotene), however, studies on the functionality of its residues are scarce. Thus, these residues are discarded without the knowledge of possible antioxidant potential. The objective of this work was to determine the stability of bioactive in soybean oil added to the extract of agroindustrial tomato waste under heating.  The extract obtained with ethyl alcohol at the ratio of 1: 5 (w/v) was initially analyzed for total phenolic compounds, lycopene, ?-carotene, and antioxidant activity using the DPPH and ?- carotene / linoleic acid. Subsequently, the extract and the synthetic antioxidants, butylhydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ) were applied in soybean oil, and the treatments subjected to heating in Rancimat (180oC/5 h). The thermoxidated samples were analyzed for the composition of tocopherols by high-performance liquid chromatography (HPLC), phytosterols, and fatty acid profile by gas chromatography (GC). The extract of agro-industrial tomato residues showed moderate results concerning its antioxidant activity, 68% by the DPPH method, and 26.2% of auto-oxidation of the ?-carotene / linoleic acid system. The synthetic antioxidant TBHQ had a greater protective effect on soybean oil when compared to the control, providing greater retention of phytosterols (85%) and tocopherols (93%). Tomato extract can be used as a natural antioxidant in industrialized oils, but with less effectiveness than the antioxidant TBHQ.
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