{"title":"原位ATP定量分析系统“IISA-ATP”的研制","authors":"N. Fukuzawa, T. Fukuba, T. Fujii","doi":"10.1109/MMB.2006.251544","DOIUrl":null,"url":null,"abstract":"An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of in situ ATP quantitative analysis system \\\"IISA-ATP\\\"\",\"authors\":\"N. Fukuzawa, T. Fukuba, T. Fujii\",\"doi\":\"10.1109/MMB.2006.251544\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected\",\"PeriodicalId\":170356,\"journal\":{\"name\":\"2006 International Conference on Microtechnologies in Medicine and Biology\",\"volume\":\"30 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2006 International Conference on Microtechnologies in Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MMB.2006.251544\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2006 International Conference on Microtechnologies in Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MMB.2006.251544","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development of in situ ATP quantitative analysis system "IISA-ATP"
An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected
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