包被支架细胞共培养在肝脏模型中的应用

O. Ramírez-Fernández, R. Godínez, E. Zuñiga-Aguilar, L. Gómez-Quiroz, M. Gutiérrez-Ruíz, J. Morales, R. Olayo
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引用次数: 2

摘要

体积细胞培养被广泛用作模拟细胞在体内组织中的特性和反应的实验模型。特别是肝细胞,是高度特化的细胞,其几何形状和与无母细胞和血管化组织的共培养对其生理和正常功能至关重要。用于细胞生长的聚合物支架已通过等离子体聚合修饰以改善其生物学特性。这项工作提出了一种聚吡咯等离子体掺杂碘(py- i)的聚l -乳酸(PLLA)涂层支架。支架中植入了两种不同的细胞系,带有星状细胞(Ito)的肝细胞(HepG2)和带有内皮细胞(HUVEC)的肝细胞(HepG2),数据与对照肝细胞(HepG2)培养进行了评估。显微镜下观察细胞形态;为了证实细胞在材料上的增殖,每周拍摄三次带有细胞的样品的图像。测定上清液中的总蛋白,结果表明,与肝细胞培养相比,内皮共培养的蛋白产量增加了25%,星状共培养的蛋白产量增加了20%。总之,我们获得了一种优化条件的Ppy-I表面共培养,以获得更好的肝脏模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell cocultures on coated scaffolds applied to liver models
Volumetric cell cultures are widely used as an experimental model to mimic the characteristics and responses of cell in tissues in vivo. Hepatocytes, particularly, are highly specialised cells, their geometrical conformation, and their coculture with no parenquimal cells and vascularised tissue, are essential to their physiology and proper function. Polymeric scaffolds for cell growth have been modified through plasma polymerisation to improve their biological properties. This work presents a Poly (L-lactic acid) (PLLA) coated scaffold with polypyrrole plasma doped with iodine (Ppy-I). The scaffold where seeded with two different cell lines, hepatocytes (HepG2) with stellate cells (Ito), and hepatocytes (HepG2) with endothelial cells (HUVEC), the data were evaluated against control hepatocytes (HepG2) culture. The morphology of the cells was evaluated by microscopy; to corroborate the cell proliferation on the material, images of the samples with the cells were taking three times per week. Total protein in the supernatant was measured, and showed that in the endothelial coculture increased 25% the protein production and the stellate coculture increased 20% the protein production against the hepatocyte culture. In conclusion, we obtain a Ppy-I surface coculture that optimises the conditions for better liver models.
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