金奈淡水水体中腕足纲(Moina brachiata, jurine, 1820)脂肪酸谱和抗氧化酶水平的比较

M. GomathiJeyam., R. Ravichandran
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摘要

多不饱和脂肪酸(PUFA)的Omega-3家族(ω-3)被认为是所有营养级动物生理功能的重要生化指标。本研究研究了海藻饲料对Adyar河和Kolavoi湖腕足鱼脂肪酸组成、抗氧化酶和DNA损伤的影响。通过气相色谱-质谱分析,鉴定出8种不同的腕足鱼脂肪酸,其中有2种PUFA(二十碳五烯酸,EPA 20:5 (ω-3);亚油酸18:2 (ω-6))。在饲喂藻类饲料的两个湖泊中,腕足小鲵的饲料脂肪酸积累和生物转化能力存在差异。以科拉沃依湖采食的腕足小鲵中ω-3 PUFA含量最高(35.84%),其次是阿德雅尔河(33.78%)。Adyar河野生腕足鱼PUFA含量显著下降(17.44%),其次是Kolavoi湖(25.78%)。另一方面,Adyar河野生臂田鼠体内丙二醛(MDA)含量升高,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH)等关键抗氧化酶水平降低,DNA损伤显著。由此可见,藻饲料可以通过降低MDA水平来提高抗氧化酶活性,而对臂田鼠没有DNA损伤。综上所述,本研究的结果表明,Scenedesmus sp.具有提高臂田鼠体内PUFA含量、抗氧化酶活性和防止DNA损伤的能力,而这种能力在野生动物中由于环境应激条件而下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of fatty acids profile and antioxidant enzyme level of cladoceran Moina brachiata (jurine, 1820) from freshwater bodies of Chennai
Omega-3 family (ω-3) of polyunsaturated fatty acids (PUFA) was considered as an important biochemical for the physiological function of all trophic level animals. In this study, we demonstrated the effect of algal diet on fatty acids composition (FA), antioxidant enzymes and DNA damage of Moina brachiata from Adyar River and Kolavoi Lake. 8 different fatty acids were identified in M. brachiata through GC-MS analysis and we noticed two PUFA (Eicosapentaenoic acid, EPA 20:5 (ω-3); Linoleic acid 18:2 (ω-6)). The dietary fatty acid accumulation and bioconversion capacity of M. brachiata have differed in two lakes fed with algal diet. The high amount of ω-3 PUFA was observed in M. brachiata fed with Scenedesmus sp. in Kolavoi Lake (35.84%) followed by Adyar River (33.78%). PUFA content was significantly declined in wild M. brachiata of Adyar River (17.44%) followed by Kolavoi lake (25.78%). On the other hand, high level of Malondialdehyde (MDA) and decreasing level of key antioxidant enzymes likes Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GSH) and DNA damage were observed in wild M. brachiata of Adyar River. Hence, the algal diet could enhance the level of antioxidant enzyme activity by decreasing the level of MDA and it does not show DNA damage on M. brachiata . Overall, the results obtained in this study explored that Scenedesmus sp., has the ability to enhance the PUFA content, antioxidant enzyme activity and prevent the DNA damage in M. brachiata which was declined in the wild animal due to the environmental stress conditions.
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