{"title":"槲皮素作为天然疗法的抗癌筛选","authors":"W. Ahmed","doi":"10.37375/sjfssu.v2i2.77","DOIUrl":null,"url":null,"abstract":"Oxidation stress is a process that damages the cells of the body, and also leads to the happen of many diseases such as cancer. This disease is known among all ages in every part of the world. In cancer cells, increasing types of free radicals flaw the balance in the cell and thus increasing free radicals target all types of molecules, including proteins, lipids, and nucleic acids. If the body cannot produce enough antioxidants, increasing free radicals damage the cells of the body. The defect in apoptosis also prevents malignant cells from being damaged. This paper aims to study the effect and activity of quercetin as an antioxidant and anticancer drug in vitro. The mechanism of increasing free radical formation causing cell damage will be explained. This study also presents a discussion about the mechanism of apoptosis pathways using the MTT scale to measure the cell's ability to metabolic activity and whether the cells are still alive. The most significant shifts were for (Raji, MOLT-4 and CT-26 equal to 0.18 ± 0.09, 2.1 ± 0.9, and 5.5 ± 0.38, respectively. Apoptosis was detected using the BD Annexin V FITC assay and apoptosis was measured at (P < 0.001). Also, in vivo, the positive effects of different doses of quercetin on affected models are discussed.","PeriodicalId":135432,"journal":{"name":"Scientific Journal for the Faculty of Science-Sirte University","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anticancer Screening of Quercetin as a Natural Treatment\",\"authors\":\"W. Ahmed\",\"doi\":\"10.37375/sjfssu.v2i2.77\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Oxidation stress is a process that damages the cells of the body, and also leads to the happen of many diseases such as cancer. This disease is known among all ages in every part of the world. In cancer cells, increasing types of free radicals flaw the balance in the cell and thus increasing free radicals target all types of molecules, including proteins, lipids, and nucleic acids. If the body cannot produce enough antioxidants, increasing free radicals damage the cells of the body. The defect in apoptosis also prevents malignant cells from being damaged. This paper aims to study the effect and activity of quercetin as an antioxidant and anticancer drug in vitro. The mechanism of increasing free radical formation causing cell damage will be explained. This study also presents a discussion about the mechanism of apoptosis pathways using the MTT scale to measure the cell's ability to metabolic activity and whether the cells are still alive. The most significant shifts were for (Raji, MOLT-4 and CT-26 equal to 0.18 ± 0.09, 2.1 ± 0.9, and 5.5 ± 0.38, respectively. Apoptosis was detected using the BD Annexin V FITC assay and apoptosis was measured at (P < 0.001). Also, in vivo, the positive effects of different doses of quercetin on affected models are discussed.\",\"PeriodicalId\":135432,\"journal\":{\"name\":\"Scientific Journal for the Faculty of Science-Sirte University\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientific Journal for the Faculty of Science-Sirte University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37375/sjfssu.v2i2.77\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific Journal for the Faculty of Science-Sirte University","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37375/sjfssu.v2i2.77","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
氧化应激是一种破坏人体细胞的过程,也会导致许多疾病的发生,如癌症。这种疾病在世界各地的所有年龄段中都是已知的。在癌细胞中,自由基类型的增加破坏了细胞内的平衡,因此增加的自由基针对所有类型的分子,包括蛋白质、脂质和核酸。如果身体不能产生足够的抗氧化剂,增加的自由基就会损害身体的细胞。细胞凋亡的缺陷也阻止了恶性细胞的破坏。本文旨在研究槲皮素作为抗氧化和抗癌药物的体外作用和活性。增加自由基形成导致细胞损伤的机制将被解释。本研究还探讨了凋亡途径的机制,利用MTT量表来衡量细胞的代谢活动能力和细胞是否仍然存活。Raji、MOLT-4和CT-26的变化幅度最大,分别为0.18±0.09、2.1±0.9和5.5±0.38。BD Annexin V FITC法检测细胞凋亡,P < 0.001)。此外,在体内,不同剂量的槲皮素对受影响的模型的积极作用进行了讨论。
Anticancer Screening of Quercetin as a Natural Treatment
Oxidation stress is a process that damages the cells of the body, and also leads to the happen of many diseases such as cancer. This disease is known among all ages in every part of the world. In cancer cells, increasing types of free radicals flaw the balance in the cell and thus increasing free radicals target all types of molecules, including proteins, lipids, and nucleic acids. If the body cannot produce enough antioxidants, increasing free radicals damage the cells of the body. The defect in apoptosis also prevents malignant cells from being damaged. This paper aims to study the effect and activity of quercetin as an antioxidant and anticancer drug in vitro. The mechanism of increasing free radical formation causing cell damage will be explained. This study also presents a discussion about the mechanism of apoptosis pathways using the MTT scale to measure the cell's ability to metabolic activity and whether the cells are still alive. The most significant shifts were for (Raji, MOLT-4 and CT-26 equal to 0.18 ± 0.09, 2.1 ± 0.9, and 5.5 ± 0.38, respectively. Apoptosis was detected using the BD Annexin V FITC assay and apoptosis was measured at (P < 0.001). Also, in vivo, the positive effects of different doses of quercetin on affected models are discussed.