{"title":"黄钩藤甲醇提取物中次生代谢产物的GC-MS和LC-MS/MS分析。茎","authors":"Astri Rozanah Siregar, Syafira Soraya, E. Sinaga","doi":"10.32996/ijbpcs.2023.5.2.1","DOIUrl":null,"url":null,"abstract":"Uncaria callophylla Blume ex Korth. is a wild plant species belonging to the Rubiaceae family. These plants thrive in the peat swamp forest of Kalimantan, Indonesia. The stems of Uncaria callophylla have been used by the Dayak Ngaju tribe to treat various diseases and to restore stamina while they work in the forest. The present study was conducted to identify the secondary metabolites in the methanolic extract of Uncaria callohylla stems to increase the knowledge about this plant and its potential in medicinal applications and drug discovery. The analysis was conducted using GC-MS and LC-MS/MS. GC-MS analysis was performed with the Agilent Technologies 7890 Gas Chromatography equipped with an Auto Sampler and 5975 Mass Selective Detector. The LC-MS analysis was carried out in the C18 column of the Waters Acquity UPLC system. MS analysis was performed with an electrospray ionization source (ESI) in positive and negative ion modes, and the compounds were identified using a UNIFI data processor with a mass spectrum library of natural active substances from the Waters Traditional Medical Scientific Library database based on UPLC/QTof MSE Data Acquisition. GC-MS analysis revealed the presence of 24 peaks, and nine of them showed quality 90% or higher, namely n-Hexadecanoic acid (16.63%), 1,3,7-Trimethyl-3,7-Dihydro-1H-Purine-2,6-Dione (13.07%), Hexadecanoic Acid, Methyl Ester; 1,2-Benzenedicarboxylic Acid, Diethyl Ester; 9,12-Octadecadienoic Acid, Methyl Ester; Methyl 14-Methylheptadecanoate; (9E,12E)-9,12-Octadecadienoic Acid; Z, E-3,13-Octadecadien-1-o1; and 2,6,10,14,18,22-Tetracosahexaene, 2,6,10,15,19,23-hexanethyl-, (all-E)-. LC-MS/MS analysis revealed the presence of five compounds, namely Arecatannin A1, Sweroside_2, Uncarine A, Epianhydrobelachinal, and Betulonic acid. All the secondary metabolites identified from GC-MS and LC-MS/MS analysis have biological activity, which indicates their medicinal potencies. From the result, it can be concluded that Uncaria callophylla stem methanolic extract contains various bioactive compounds justifies its traditional use, and therefore it can be developed further for new drug discovery.","PeriodicalId":127127,"journal":{"name":"International Journal of Biological, Physical and Chemical Studies","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"GC-MS and LC-MS/MS Analysis of Secondary Metabolites in the Methanolic Extract of Uncaria callophylla Blume ex Korth. Stems\",\"authors\":\"Astri Rozanah Siregar, Syafira Soraya, E. Sinaga\",\"doi\":\"10.32996/ijbpcs.2023.5.2.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Uncaria callophylla Blume ex Korth. is a wild plant species belonging to the Rubiaceae family. These plants thrive in the peat swamp forest of Kalimantan, Indonesia. The stems of Uncaria callophylla have been used by the Dayak Ngaju tribe to treat various diseases and to restore stamina while they work in the forest. The present study was conducted to identify the secondary metabolites in the methanolic extract of Uncaria callohylla stems to increase the knowledge about this plant and its potential in medicinal applications and drug discovery. The analysis was conducted using GC-MS and LC-MS/MS. GC-MS analysis was performed with the Agilent Technologies 7890 Gas Chromatography equipped with an Auto Sampler and 5975 Mass Selective Detector. The LC-MS analysis was carried out in the C18 column of the Waters Acquity UPLC system. MS analysis was performed with an electrospray ionization source (ESI) in positive and negative ion modes, and the compounds were identified using a UNIFI data processor with a mass spectrum library of natural active substances from the Waters Traditional Medical Scientific Library database based on UPLC/QTof MSE Data Acquisition. GC-MS analysis revealed the presence of 24 peaks, and nine of them showed quality 90% or higher, namely n-Hexadecanoic acid (16.63%), 1,3,7-Trimethyl-3,7-Dihydro-1H-Purine-2,6-Dione (13.07%), Hexadecanoic Acid, Methyl Ester; 1,2-Benzenedicarboxylic Acid, Diethyl Ester; 9,12-Octadecadienoic Acid, Methyl Ester; Methyl 14-Methylheptadecanoate; (9E,12E)-9,12-Octadecadienoic Acid; Z, E-3,13-Octadecadien-1-o1; and 2,6,10,14,18,22-Tetracosahexaene, 2,6,10,15,19,23-hexanethyl-, (all-E)-. LC-MS/MS analysis revealed the presence of five compounds, namely Arecatannin A1, Sweroside_2, Uncarine A, Epianhydrobelachinal, and Betulonic acid. All the secondary metabolites identified from GC-MS and LC-MS/MS analysis have biological activity, which indicates their medicinal potencies. From the result, it can be concluded that Uncaria callophylla stem methanolic extract contains various bioactive compounds justifies its traditional use, and therefore it can be developed further for new drug discovery.\",\"PeriodicalId\":127127,\"journal\":{\"name\":\"International Journal of Biological, Physical and Chemical Studies\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological, Physical and Chemical Studies\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32996/ijbpcs.2023.5.2.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological, Physical and Chemical Studies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32996/ijbpcs.2023.5.2.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
鸡翅钩藤(Uncaria callophylla Blume)是茜草科的一种野生植物。这些植物在印度尼西亚加里曼丹的泥炭沼泽森林中茁壮成长。在达雅克·恩加朱部落,当他们在森林里工作时,钩藤的茎被用来治疗各种疾病和恢复体力。本研究旨在鉴定钩藤(Uncaria callohylla)茎甲醇提取物中的次生代谢物,以增加对该植物的认识及其在药用和药物开发方面的潜力。采用GC-MS和LC-MS/MS进行分析。采用配备自动进样器和5975质量选择检测器的Agilent Technologies 7890气相色谱仪进行GC-MS分析。采用Waters Acquity UPLC系统的C18柱进行LC-MS分析。采用电喷雾电离源(ESI)在正离子和负离子模式下进行质谱分析,采用UNIFI数据处理器和基于UPLC/QTof MSE数据采集的Waters Traditional Medical Scientific library数据库中的天然活性物质质谱库对化合物进行鉴定。GC-MS分析共发现24个峰,其中9个峰的质量为90%及以上,分别为正十六酸(16.63%)、1,3,7-三甲基-3,7-二氢- 1h -嘌呤-2,6-二酮(13.07%)、十六酸甲酯;1,2-苯二甲酸二乙酯;9,12-十八二烯酸甲酯;甲基14-Methylheptadecanoate;(9 e, 12 e) 9日12-Octadecadienoic酸;13-Octadecadien-1-o1 Z, e - 3;2,6、10、14、18、22-四碳六烯,2,6、10、15、19、23-己基-,(全e)-。LC-MS/MS分析结果显示,其中含有槟酒素A1、Sweroside_2、Uncarine A、Epianhydrobelachinal和桦木酸5种化合物。GC-MS和LC-MS/MS分析鉴定的次生代谢物均具有生物活性,说明其具有一定的药用价值。结果表明,甲壳钩藤茎甲醇提取物中含有多种生物活性成分,证明了其传统用途是合理的,因此可以进一步开发用于新药开发。
GC-MS and LC-MS/MS Analysis of Secondary Metabolites in the Methanolic Extract of Uncaria callophylla Blume ex Korth. Stems
Uncaria callophylla Blume ex Korth. is a wild plant species belonging to the Rubiaceae family. These plants thrive in the peat swamp forest of Kalimantan, Indonesia. The stems of Uncaria callophylla have been used by the Dayak Ngaju tribe to treat various diseases and to restore stamina while they work in the forest. The present study was conducted to identify the secondary metabolites in the methanolic extract of Uncaria callohylla stems to increase the knowledge about this plant and its potential in medicinal applications and drug discovery. The analysis was conducted using GC-MS and LC-MS/MS. GC-MS analysis was performed with the Agilent Technologies 7890 Gas Chromatography equipped with an Auto Sampler and 5975 Mass Selective Detector. The LC-MS analysis was carried out in the C18 column of the Waters Acquity UPLC system. MS analysis was performed with an electrospray ionization source (ESI) in positive and negative ion modes, and the compounds were identified using a UNIFI data processor with a mass spectrum library of natural active substances from the Waters Traditional Medical Scientific Library database based on UPLC/QTof MSE Data Acquisition. GC-MS analysis revealed the presence of 24 peaks, and nine of them showed quality 90% or higher, namely n-Hexadecanoic acid (16.63%), 1,3,7-Trimethyl-3,7-Dihydro-1H-Purine-2,6-Dione (13.07%), Hexadecanoic Acid, Methyl Ester; 1,2-Benzenedicarboxylic Acid, Diethyl Ester; 9,12-Octadecadienoic Acid, Methyl Ester; Methyl 14-Methylheptadecanoate; (9E,12E)-9,12-Octadecadienoic Acid; Z, E-3,13-Octadecadien-1-o1; and 2,6,10,14,18,22-Tetracosahexaene, 2,6,10,15,19,23-hexanethyl-, (all-E)-. LC-MS/MS analysis revealed the presence of five compounds, namely Arecatannin A1, Sweroside_2, Uncarine A, Epianhydrobelachinal, and Betulonic acid. All the secondary metabolites identified from GC-MS and LC-MS/MS analysis have biological activity, which indicates their medicinal potencies. From the result, it can be concluded that Uncaria callophylla stem methanolic extract contains various bioactive compounds justifies its traditional use, and therefore it can be developed further for new drug discovery.