不同载体材料对几种昆虫病原真菌液体生物农药活性和分生孢子密度的影响

U. Triasih, D. Agustina, M. E. Dwiastuti, S. Wuryantini
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引用次数: 2

摘要

印度尼西亚是一个拥有大量生物多样性的国家,但尚未充分利用于农业。生物制剂防治病虫害的有效性还有待进一步研究,其中之一就是利用昆虫病原真菌作为生物农药。本研究的目的是确定贮藏对几种昆虫病原真菌分生孢子活力和密度的影响;球孢白僵菌,绿僵菌,拟青霉。本研究选用5株昆虫病原真菌,2株球孢白僵菌(JBG和JBR), 2株绿僵菌(JBG和UST), 1株拟青霉。生物农药载体材料由6种载体材料组成,分别为大豆煎煮废液、大豆洗涤废液、马铃薯葡萄糖琼脂、木薯淀粉废液、大米废液、大米废液和椰子水。生物农药在一年前制成,一年后重新检查分生孢子的活力和复杂性。通过对分生孢子密度的观察,发现球孢白僵菌JBG液体生物农药的分生孢子密度为4.8 × 10 ×分生孢子/ ml,而大豆汤(RK)载体的初始密度为3.4 × 10⁸/ ml。金龟子甲基化菌在大豆洗涤废渣上的存活率最高,为14%。关键词:分生孢子活力,分生孢子密度,昆虫病原真菌
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Test of Various Carrier Materials Against Viability and Conidia Density in Some Liquid Biopesticides of Entomopathogenic Fungi
Indonesia is a country that has a large biological diversity but has not been fully utilized for agriculture. The effectiveness of biological agents in controlling pests and plant diseases still needs further investigation, one of which is the use of entomopathogenic fungi as biopesticides. The purpose of this study was to determine the effect of storage on the viability and density of conidia in several entomopathogenic fungi; Beauveria bassiana, Metarhizium anisopliae, Paecilomyces. This study used 5 entomopathogenic fungi isolates, 2 Beauveria bassiana isolates (JBG and JBR), 2 Metarhizium anisopliae isolates (JBG and UST), 1 Paecilomyces. Biopesticide carrier material consisted of 6 carrier materials, namely soybean decoction waste, soy washing waste, Potato Dextrose Agar, tapioca waste, rice waste, rice washing waste and coconut water. Biopesticides have been made one year before and the viability and complexity of the conidia is re-examined one year later. From the observations of conidial density, the best was found in Beauveria bassiana JBG liquid biopesticide of 4.8 x 10 x conidia / ml from the initial density of 3.4 x 10⁸ / ml in the soybean decoction (RK) carrier material. The highest viability was found in JBG Metharizium anisopliae on soybean washing waste material by 14%. Key words: Conidia viability, conidial density, entomopathogenic fungi
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