光照对培养小鼠卵发育的影响

Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ Pub Date : 1993-12-01 DOI:10.1109/IEMBS.1993.979316

H. Itoh, N. Makimura, T. Arai, H. Tsubamoto, K. Furuya, M. Kikuchi, I. Nagata

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引用次数: 1

摘要

在体外研究了光照环境对小鼠卵发育的影响。用波长分别为632.8nm、543nm和442nm的激光对1细胞小鼠卵进行体外照射。本实验通过显微镜光源测量体外受精胚胎移植(IVFET)的实际光环境，以确定体外受精胚胎移植的光剂量。培养小鼠卵，观察细胞发育阶段。在500kJ/m2的632.8nm辐射(比实际光环境大20倍)下，97%的卵停止分裂。在543nm和442nm辐射下，对卵细胞分裂无影响。这些结果表明，光环境可能对小鼠卵子的体外发育有影响。

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Influence of light irradiation an the development of mouse eggs in culture

We tnvestlgated the Influence of l lght envlronment on the development of mouse egg in virro. The 1-cell mouse eggs were irradlated by varlous laser l ights of whlch wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual llght environment by means of the microscope IIght source In IVFET ( in vitro fertlllzatlon-embryo transfer) was measured to set the I lght dose In th is experlment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500kJ/m2 I n dose ( 2 0 t lmes larger than actual l lght envlronment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed In egg division. These results suggested that l lght environment migh t af fect o n the development of mouse egg in vitro.

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Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ

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