Use of RAPD Markers to Analyze Genetic Variability of Introgressed Brassica Lines

Seven individuals of introgressed Brassica lines (Binasarisha-5/Daulot) and two of their parental lines were used for this study to estimate genetic variability using three randomly amplified polymorphic DNA (RAPD) markers (61AB10G1, 72AB10G12 and 73AB10T13). A total of 23 clear bands were scored, of which 21 (91.30%) bands were proved to be polymorphic. The highest proportion of polymorphic loci and gene diversity values were 43.48% and 0.187, respectively in the line five of Binasarisha-5/Daulot. The lower proportion of polymorphic loci and gene diversity values were 8.70% and 0.034; 8.70% and 0.026 in the line seven of the cross and one parent, Daulot, respectively. The co-efficient of gene differentiation (G st ) and gene flow (N m ) values were 0.677 and 0.237, found respectively from the Popgene analysis. Result of cluster analysis indicated that the nine accessions were capable of being classified into two major groups - one consists of only one parent Daulot ( Brassica juncea ) while another consists of Binasarisha-5 ( Brassica napus ) and all introgressed lines of C 6 generation (treated with colchicine in C 1 generation) resulted from the cross B. napus and B. juncea . Introgressed line seven and Binasarisha-5 showed the lowest genetic distance of 0.077. Higher similarity was found between Binasarisha-5 and introgressed progenies. Introgressed line one and Daulot showed the highest genetic distance of 0.709, which can be used as germplasm for breeding program that aim to improve Brassica . It was concluded that RAPD markers can be used for the study of molecular characterization and diversity in Brassica . The Agriculturists 2016; 14(1) 122-133