Nugraheni Hadiyanti, S. Supriyadi, Pardono Pardono
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引用次数: 4

摘要

枸杞(Physalis spp.)是一种具有潜在药用价值的野生植物。有关印尼Physalis spp.多样性的资料有限。从形态特征、总蛋白谱和黄酮类化合物等方面对东爪哇克鲁德山野生Physalis sp.的多样性进行了研究。研究在海拔200-400、400-600、600-800、800 - 1000和> 1000 m的梯度上进行了调查。形态学性状鉴定主要集中在根、茎、叶、花、果等16个性状上进行评分。采用SDS-PAGE法鉴定总蛋白谱,根据比色法原理,采用紫外分光光度法对黄酮类化合物进行分析。通过聚类分析,利用形态相似性和总蛋白谱来确定Physalis sp .的多样性程度。结果表明,在海拔1000 m以上的克鲁德岛上均有Physalis sp .。海拔对其形态多样性、总蛋白谱和黄酮类化合物均有影响。对Physalis sp.多样性起作用的形态学特征有:茎的生长方向、叶形状、叶缘、叶表面、叶柄颜色、花内颈染色、果萼颜色。总蛋白的表达。在低梯度下,与中、高梯度相比,黄酮类化合物含量较高。在东爪哇克鲁德山的坡度上发现了两种Physalis,分别是Physalis minima L.和Physalis angulata L.。
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KERAGAMAN BEBERAPA TUMBUHAN CIPLUKAN (Physalis spp.) DI LERENG GUNUNG KELUD, JAWA TIMUR
Ciplukan (Physalis spp.) is a wild plant which is that potentially used as a medicinal plant. Information related to the diversity of Physalis spp. in Indonesia is limited. The objectives of the research was to identify the diversity of wild Physalis sp. on Mt. Kelud, East Java based on morphological characters, total protein profiles, and flavonoid compounds. The research was conducted by survey on some gradients viz. 200–400, 400–600, 600–800, 800–1,000 and >1,000 m above sea level. Identification of morphological characters was focused on root, stem, leaf, flower and fruit covering 16 characters for scoring. Identification of total protein profile was performed by SDS-PAGE method and the flavonoid compound was analyzed using UV spectrophotometric method based on the colorimetric principle. The degree of diversity of Physalis spp. was determined based on morphological similarity and total protein profiles through cluster analysis. The results showed that Physalis spp. on Kelud was found up to 1,000 m above sea level. Altitude affected to the morphological diversity, total protein profile and the flavonoid compound. Morphological features that play a role in the diversity of Physalis sp. were the direction of growing stems, leaf shape, leaf margin, leaf surface, leaf stalk color, stain color on the inner neck of thr flower, and fruiting calyx color. Expression of total protein P. minima. shows similarity to P. angulata L. Physalis sp. in the low gradient have a higher flavonoid compound as compared to the middle and high gradients. There were two species of Physalis found on the gradient of Mt. Kelud, East Java, namely Physalis minima L. and Physalis angulata L.
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