P J van der Kooij, J P Persijn, W van der Slik, C B Korsten
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引用次数: 4
摘要
本文建立了一种测定血清核苷酸酶(EC 3.1.3.5)的新方法。该试验以胞苷-5′-单磷酸作为底物，然后对生成的胞苷进行脱胺。该方法的原理与用5′-单磷酸腺苷作底物的Persijn- van der Slik法测定Berthelot吲哚酚反应中释放的氨相当。两种方法所得结果具有良好的相关性;该方法具有灵敏度高的优点。
Determination of serum nucleotidase with cytidine monophosphate as substrate, (I).
This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity.
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