Selective Enrichment Methods for N-Glycosite Containing Peptides in N-Glycoproteomics

Glycosylation is an essential biochemical process. It is of great significance to detect the glycoproteins sensitively to understand its biological function. While the rapid development of MS-based technologies has considerably promoted the research of glycoproteome, the low abundance and heterogeneity of glycosylation still make the comprehensive analysis of glycoproteome challenging. To analyze the glycoproteome with high selectivity and sensitivity, the enrichment process before mass spectrometry (MS) analysis is particularly critical. In this chapter, current representative methods for glycoproteins/glycopeptides enrichment are summarized with advantages and limitations discussed.