XI Simposio, R. Argentina, Libro de Resúmenes, Alberto Bozzo, Alejandro Henry, R. Secco
{"title":"第十届拉丁美洲和加勒比农业生物技术会议和第十一届阿根廷REDBIO研讨会。摘要书。","authors":"XI Simposio, R. Argentina, Libro de Resúmenes, Alberto Bozzo, Alejandro Henry, R. Secco","doi":"10.35676/inia/st.253","DOIUrl":null,"url":null,"abstract":"Intensive exploitation of mahogany wood (Swietenia macrophylla, Meliaceae) has resulted in the loss of natural populations. Somatic embryogenesis offers an alternative to clonal propagation and conservation of mahogany. This study describes biochemical (carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators content) and histological characteristics of the somatic embryogenesis process in mahogany. Calli were obtained by culturing cotyledons of seeds from immature fruits for six weeks on semisolid MS medium supplemented with 1.0 mgL-1 of kinetin and 4.0 mgL-1 of 2, 4-D. Primary callus was cultured on half-strength semi-solid MS medium supplemented with 1.0 mgl-1 6BA (6-benzylaminopurine) and embryogenic structures were obtained. Embryo development from globular-shaped somatic embryos to the cotyledonary stage was confirmed by histology and scanning electron microscopy. Shoot initiation was observed after somatic embryos were transferred to the germination and maturation medium. Endogenous concentrations of carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators were determined in embryogenic (EC) and non-embryogenic (NEC) calli of mahogany. Embryogenic cultures contained significantly higher concentrations of IAA (indoleacetic acid), ABA (abscisic acid), and GAs (Gibberellins 1+3+20), whereas nonembryogenic calli contained more total phenols, flavonoids, and resistant starch. Fructose and glucose were not present at detectable levels in EC or NEC, whereas soluble starch and sucrose were only detectable in EC. Concentrations of total proteins, Z/ZR (Zeatin/zeatin riboside) and iP/iPA (N6-(∆2-isopentenyl) adenine and N6-(∆2-isopentenyl) adenosine) were similar in EC and NEC. 1 Laboratorio de Biotecnología de Plantas, Escuela de Biología, Universidad de Costa Rica; Costa Rica 2 Escuela de Biología, Universidad de Costa Rica, Costa Rica 3 Centro de Investigación en Estructuras Microscópicas (CIEMIC), Universidad de Costa Rica, Costa Rica 4 Laboratorio de Fitofarmacología, Tecnología Farmacéutica y Cosmética (LAFITEC), Instituto de Investigaciones Farmacéuticas, Universidad de Costa Rica, Costa Rica 5. Institute of Crop Science, University of Hohenheim, Germany","PeriodicalId":418664,"journal":{"name":"INIA Serie técnica","volume":"11 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"X ENCUENTRO LATINOAMERICANO Y DEL CARIBE DE BIOTECNOLOGÍA AGROPECUARIA Y XI SIMPOSIO REDBIO ARGENTINA. Libro de Resúmenes.\",\"authors\":\"XI Simposio, R. Argentina, Libro de Resúmenes, Alberto Bozzo, Alejandro Henry, R. Secco\",\"doi\":\"10.35676/inia/st.253\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Intensive exploitation of mahogany wood (Swietenia macrophylla, Meliaceae) has resulted in the loss of natural populations. Somatic embryogenesis offers an alternative to clonal propagation and conservation of mahogany. This study describes biochemical (carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators content) and histological characteristics of the somatic embryogenesis process in mahogany. Calli were obtained by culturing cotyledons of seeds from immature fruits for six weeks on semisolid MS medium supplemented with 1.0 mgL-1 of kinetin and 4.0 mgL-1 of 2, 4-D. Primary callus was cultured on half-strength semi-solid MS medium supplemented with 1.0 mgl-1 6BA (6-benzylaminopurine) and embryogenic structures were obtained. Embryo development from globular-shaped somatic embryos to the cotyledonary stage was confirmed by histology and scanning electron microscopy. Shoot initiation was observed after somatic embryos were transferred to the germination and maturation medium. Endogenous concentrations of carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators were determined in embryogenic (EC) and non-embryogenic (NEC) calli of mahogany. Embryogenic cultures contained significantly higher concentrations of IAA (indoleacetic acid), ABA (abscisic acid), and GAs (Gibberellins 1+3+20), whereas nonembryogenic calli contained more total phenols, flavonoids, and resistant starch. Fructose and glucose were not present at detectable levels in EC or NEC, whereas soluble starch and sucrose were only detectable in EC. Concentrations of total proteins, Z/ZR (Zeatin/zeatin riboside) and iP/iPA (N6-(∆2-isopentenyl) adenine and N6-(∆2-isopentenyl) adenosine) were similar in EC and NEC. 1 Laboratorio de Biotecnología de Plantas, Escuela de Biología, Universidad de Costa Rica; Costa Rica 2 Escuela de Biología, Universidad de Costa Rica, Costa Rica 3 Centro de Investigación en Estructuras Microscópicas (CIEMIC), Universidad de Costa Rica, Costa Rica 4 Laboratorio de Fitofarmacología, Tecnología Farmacéutica y Cosmética (LAFITEC), Instituto de Investigaciones Farmacéuticas, Universidad de Costa Rica, Costa Rica 5. Institute of Crop Science, University of Hohenheim, Germany\",\"PeriodicalId\":418664,\"journal\":{\"name\":\"INIA Serie técnica\",\"volume\":\"11 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"INIA Serie técnica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35676/inia/st.253\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"INIA Serie técnica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35676/inia/st.253","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
X ENCUENTRO LATINOAMERICANO Y DEL CARIBE DE BIOTECNOLOGÍA AGROPECUARIA Y XI SIMPOSIO REDBIO ARGENTINA. Libro de Resúmenes.
Intensive exploitation of mahogany wood (Swietenia macrophylla, Meliaceae) has resulted in the loss of natural populations. Somatic embryogenesis offers an alternative to clonal propagation and conservation of mahogany. This study describes biochemical (carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators content) and histological characteristics of the somatic embryogenesis process in mahogany. Calli were obtained by culturing cotyledons of seeds from immature fruits for six weeks on semisolid MS medium supplemented with 1.0 mgL-1 of kinetin and 4.0 mgL-1 of 2, 4-D. Primary callus was cultured on half-strength semi-solid MS medium supplemented with 1.0 mgl-1 6BA (6-benzylaminopurine) and embryogenic structures were obtained. Embryo development from globular-shaped somatic embryos to the cotyledonary stage was confirmed by histology and scanning electron microscopy. Shoot initiation was observed after somatic embryos were transferred to the germination and maturation medium. Endogenous concentrations of carbohydrates, total phenols, total flavonoids, protein, and plant growth regulators were determined in embryogenic (EC) and non-embryogenic (NEC) calli of mahogany. Embryogenic cultures contained significantly higher concentrations of IAA (indoleacetic acid), ABA (abscisic acid), and GAs (Gibberellins 1+3+20), whereas nonembryogenic calli contained more total phenols, flavonoids, and resistant starch. Fructose and glucose were not present at detectable levels in EC or NEC, whereas soluble starch and sucrose were only detectable in EC. Concentrations of total proteins, Z/ZR (Zeatin/zeatin riboside) and iP/iPA (N6-(∆2-isopentenyl) adenine and N6-(∆2-isopentenyl) adenosine) were similar in EC and NEC. 1 Laboratorio de Biotecnología de Plantas, Escuela de Biología, Universidad de Costa Rica; Costa Rica 2 Escuela de Biología, Universidad de Costa Rica, Costa Rica 3 Centro de Investigación en Estructuras Microscópicas (CIEMIC), Universidad de Costa Rica, Costa Rica 4 Laboratorio de Fitofarmacología, Tecnología Farmacéutica y Cosmética (LAFITEC), Instituto de Investigaciones Farmacéuticas, Universidad de Costa Rica, Costa Rica 5. Institute of Crop Science, University of Hohenheim, Germany
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
