{"title":"基于微流控芯片的条纹荧光传感器非接触测量单个卵母细胞耗氧量","authors":"Masanobu Kito, H. Maruyama, F. Arai","doi":"10.1109/MHS.2014.7006137","DOIUrl":null,"url":null,"abstract":"We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip\",\"authors\":\"Masanobu Kito, H. Maruyama, F. Arai\",\"doi\":\"10.1109/MHS.2014.7006137\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.\",\"PeriodicalId\":181514,\"journal\":{\"name\":\"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"volume\":\"56 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MHS.2014.7006137\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2014.7006137","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip
We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.
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