{"title":"一种通过荧光检测的水中大肠杆菌筛选机","authors":"Aniwat Juhong, W. Piyawattanametha","doi":"10.1109/BMEICON.2018.8609922","DOIUrl":null,"url":null,"abstract":"We have developed a fluorescence based detection for rapid E. coli which is an indicator for fecal contamination for water quality. This technology has cut down the time taken to detect E. coli from a few days to just a couple of hours. The system relies on the enzyme β-D glucuronidase (GUD) reacts with 4-methylumblliferyl-β-D glucuronide (MUG) resulting in a byproduct named 4-methylumbellliferone (4MU). Our system can detect this byproduct in the concentration range from 0.01 to 80 μM which can then be enumerated to be the number of E. coli.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"30 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An E. coli screening machine in water via fluorescence detection\",\"authors\":\"Aniwat Juhong, W. Piyawattanametha\",\"doi\":\"10.1109/BMEICON.2018.8609922\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have developed a fluorescence based detection for rapid E. coli which is an indicator for fecal contamination for water quality. This technology has cut down the time taken to detect E. coli from a few days to just a couple of hours. The system relies on the enzyme β-D glucuronidase (GUD) reacts with 4-methylumblliferyl-β-D glucuronide (MUG) resulting in a byproduct named 4-methylumbellliferone (4MU). Our system can detect this byproduct in the concentration range from 0.01 to 80 μM which can then be enumerated to be the number of E. coli.\",\"PeriodicalId\":232271,\"journal\":{\"name\":\"2018 11th Biomedical Engineering International Conference (BMEiCON)\",\"volume\":\"30 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2018 11th Biomedical Engineering International Conference (BMEiCON)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/BMEICON.2018.8609922\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2018 11th Biomedical Engineering International Conference (BMEiCON)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/BMEICON.2018.8609922","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
An E. coli screening machine in water via fluorescence detection
We have developed a fluorescence based detection for rapid E. coli which is an indicator for fecal contamination for water quality. This technology has cut down the time taken to detect E. coli from a few days to just a couple of hours. The system relies on the enzyme β-D glucuronidase (GUD) reacts with 4-methylumblliferyl-β-D glucuronide (MUG) resulting in a byproduct named 4-methylumbellliferone (4MU). Our system can detect this byproduct in the concentration range from 0.01 to 80 μM which can then be enumerated to be the number of E. coli.
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