差示共聚焦显微镜的图像绘制

Lirong Qiu, Lei Wang, Dali Liu, Maosheng Hou, Weiqian Zhao
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引用次数: 0

摘要

差示共聚焦显微镜在过零触发测量过程中,样品表面特征或倾斜会导致边缘无法触发。同时,环境振动也会引起误触发。为了恢复样本的无效信息,实现高精度的表面形貌测量,采用全变分(TV)模型对扫描图像进行恢复。对该方法进行了仿真分析和实验验证。基于TV模型的图像修复算法通过变分法解决了能量方程的最小化问题,能够有效地恢复带有噪声的无纹理图像。利用该算法恢复了模拟共聚焦激光的强度曲线和标准阶跃样品的高度曲线。修复阈值以下的强度曲线后,与理想情况的最大偏差为0.0042,恢复高度曲线对应的边缘轮廓,最大偏差为0.1920,证明了算法的有效性。光栅修补实验表明,在差共聚焦显微镜过零触发测量中,TV算法可以恢复触发失败造成的丢失信息和消除误触发造成的噪声。恢复后的图像与OLYMPUS共聚焦显微镜的扫描结果一致,可以满足后续测量分析的要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Image inpainting for the differential confocal microscope
In the process of zero-crossing trigger measurement of differential confocal microscope, the sample surface features or tilt will cause the edges can't be triggered. Meanwhile, environment vibration can also cause false triggering. In order to restore the invalid information of sample, and realize high-precision surface topography measurement, Total Variation (TV) inpainting model is applied to restore the scanning images. Emulation analysis and experimental verification of this method are investigated. The image inpainting algorithm based on TV model solves the minimization of the energy equation by calculus of variations, and it can effectively restore the non-textured image with noises. Using this algorithm, the simulation confocal laser intensity curve and height curve of standard step sample are restored. After inpainting the intensity curve below the threshold is repaired, the maximum deviation from ideal situation is 0.0042, the corresponding edge contour of height curve is restored, the maximum deviation is 0.1920, which proves the algorithm is effective. Experiment of grating inpainting indicates that the TV algorithm can restore the lost information caused by failed triggering and eliminate the noise caused by false triggering in zero-crossing trigger measurement of differential confocal microscope. The restored image is consistent with the scanning result of OLYMPUS confocal microscope, which can satisfy the request of follow-up measurement analysis.
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