部分药用植物叶面潜在致病菌的分离

HY Ismail, H. Bello, I. Allamin, E. Danjuma
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引用次数: 1

摘要

微生物无处不在,它们的影响可以直接或间接地得到认识。本研究旨在测定一些常用口服治疗疾病的药用植物叶面致病菌的发生情况。无菌采集决明子(Cassia gusia)、芒果(Mangifera indica)和番石榴(Psidium guajavum)叶片样品,并对其细菌含量进行测定。在原始样品中观察到较高的细菌计数,然后用无菌水洗涤。用无菌温水(焯水)洗涤的样品计数较低,但无统计学意义(p≤0.05)。生料中的番石榴叶(3.4×106 cfu/g)和焯水后的决明子叶(6.4×105 cfu/g)计数较高。两种处理中芒果叶的cfu/g最低,分别为8.4×105和2.0×105。分离到革兰氏阴性菌和革兰氏阳性菌共20种。细菌鉴定结果显示,常见菌种为奇异变形杆菌(35%)、寻常变形杆菌(15%)、大肠杆菌(15%)、肺炎克雷伯菌(5%)、摩根氏菌(5%)、伤寒沙门菌(10%)、肠杆菌(5%)、金黄色葡萄球菌(5%)和霍乱弧菌(5%)。这些微生物可能是潜在的病原体,用热水适当清洗可能是减少微生物含量的更好方法,因此,建议这样做。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation of potential bacterial pathogens from the phylloplane of some selected medicinal plants
Microorganisms are ubiquitous and their impact could be appreciated directly or indirectly. This study was aimed at determining the occurrence of pathogenic bacteria on the phylloplane of some medicinal plants commonly used to treat diseases by oral administration. Leaves samples were collected aseptically from Cassia fistula (Cassia) Mangifera indica (Mango) and Psidium guajavum (Guava) and their bacterial contents were assessed. Higher bacterial counts were observed in raw samples followed by those washed with sterile water. Samples washed with sterile warm water (blanched) were shown to have lower counts although without statistical significance (p ≤ 0.05). Guava leaves had higher counts (3.4×106 cfu/g) in the raw samples and cassia in blanched (6.4×105 cfu/g). However, mango leaves had the lowest (8.4×105 cfu/g and 2.0×105 cfu/g) in both the treatments respectively. Twenty bacterial species were isolated comprising Gram negative and Gram positive species. Bacterial identification revealed that Proteus mirabilis (35%), Proteus vulgaris (15%), Escherichia coli (15%), Klebsiella pneumoniae (5%), Morganella morganii (5%), Salmonella typhi (10%), Enterobacter sp. (5%), Staphylococcus aureus (5%) and Vibrio cholarae (5%) were the prevalent species. These organisms could be potential pathogens and proper washing with hot water may serve a better means of reducing the microbial contents and thus, it is recommended.
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