新型基于生物芯片的核酸分子绝对定量检测系统:初级试验

Jie Gao, Z. Ji, Lei Zhang, Z. Shao, Yong-ping Yan
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引用次数: 0

摘要

核酸分子绝对定量检测系统的研究是分子生物学中一个引人注目的课题。许多大企业仍在继续投资于数字PCR等此类技术的研究和开发。我们设计了一种新的基于生物芯片的绝对定量检测系统,该系统将聚合物生物芯片与算法计算相结合,以确定临床样品中HBV-DNA的绝对定量浓度。我们的研究结果表明,利用生物芯片和计算机辅助系统构建廉价的便携式绝对qPCR装置是很有可能的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New Biochip-Based Absolute Quantitative Detection System for Nucleic Acid Molecules: A Primary Assay
Absolute quantitative detection system study for nucleic acid molecules is an eye-catching subject in molecular biology. Many large companies are continuing to pour money into research and development of this kind technology such as digital PCR. We demonstrate here for a new design biochip-based absolute quantitative detection system, in which polymer biochip combines with algorithm computation to find out absolute quantitative concentration for HBV-DNA in clinical samples. Our results show that the way to construction of inexpensive portable device for absolute qPCR is highly possible with biochip-based and computer-aided system.
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