波导倏逝场显微镜在细胞和细菌生物物理学中的应用

2016 4th International Conference on Photonics, Optics and Laser Technology (PHOTOPTICS) Pub Date : 2016-12-13 DOI:10.5220/0005618302010212

S. Mittler

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引用次数: 0

摘要

提出了基于永久耦合光栅的玻璃板波导的两种倏逝场显微镜技术:波导倏逝场荧光显微镜(WEFF)和波导倏逝场散射显微镜(WEFS)。简要介绍了实验技术:实验装置是基于传统的倒置显微镜。对TIR和TIRF显微镜进行了比较。波导方法的优点得到了明确的阐述。给出了WEFF和WEFS显微镜的各种例子。WEFF:使用多模波导的静态距离映射，细胞质膜的动态溶解研究和成骨细胞对胰蛋白酶的动力学响应。用于WEFS:细菌灭菌以及细胞粘附和粒度研究。最新的发展是一种可批量生产的全聚合物波导芯片，将该技术引入感兴趣的科学界。

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Waveguide evanescent field microscopies for application in cell- and bacteria-biophysics

Two evanescent field microscopy technologies based on glass slab waveguides with permanent coupling gratings are presented: waveguide evanescent field fluorescence microscopy (WEFF) and waveguide evanescent field scattering microscopy (WEFS). The technologies are briefly described: the experimental setup is based on a conventional inverted microscope. A comparison to TIR and TIRF microscopy is given. The advantages of the waveguide method are clearly addressed. Various examples from for WEFF and WEFS microscopy are given. For WEFF: static distance mapping with a multimode waveguide, dynamic solubilisation studies of cell plasma membranes and the kinetic response of osteoblasts to trypsin. For WEFS: bacteria sterilization as well as cell adhesion and granularity studies. The latest development is a mass producible all-polymer-waveguide-chip to bring the technology to the interested scientific community.

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来源期刊

2016 4th International Conference on Photonics, Optics and Laser Technology (PHOTOPTICS)

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