{"title":"对虾样品中副溶血性弧菌的检测及分子特性研究","authors":"Daryoush Asgarpoor, F. Haghi, H. Zeighami","doi":"10.2174/1874070701812010046","DOIUrl":null,"url":null,"abstract":"Methods: A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp–toxR,tdh and trh genes.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"58 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples\",\"authors\":\"Daryoush Asgarpoor, F. Haghi, H. Zeighami\",\"doi\":\"10.2174/1874070701812010046\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Methods: A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp–toxR,tdh and trh genes.\",\"PeriodicalId\":296126,\"journal\":{\"name\":\"The Open Biotechnology Journal\",\"volume\":\"58 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Open Biotechnology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874070701812010046\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Biotechnology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874070701812010046","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples
Methods: A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp–toxR,tdh and trh genes.
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