{"title":"药用植物枇杷内生细菌的生物化学鉴定","authors":"E. Astuty, Indas Wari Rahman, M. Yunita","doi":"10.31932/jpbio.v8i1.2095","DOIUrl":null,"url":null,"abstract":"Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.","PeriodicalId":124686,"journal":{"name":"JPBIO (Jurnal Pendidikan Biologi)","volume":"105 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biochemical identification of endophytic bacteria isolates from medicinal plant kayu jawa lannea coromandelica (houtt.) merr\",\"authors\":\"E. Astuty, Indas Wari Rahman, M. Yunita\",\"doi\":\"10.31932/jpbio.v8i1.2095\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.\",\"PeriodicalId\":124686,\"journal\":{\"name\":\"JPBIO (Jurnal Pendidikan Biologi)\",\"volume\":\"105 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JPBIO (Jurnal Pendidikan Biologi)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31932/jpbio.v8i1.2095\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JPBIO (Jurnal Pendidikan Biologi)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31932/jpbio.v8i1.2095","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
药用植物及其内生菌是有价值的生物活性化合物及其次生代谢产物的来源,占市场上可获得的天然药物的80%以上。长期以来,Kayu Jawa (Lannea coromandelica (Houtt) Merr)一直被用作传统的治疗草药。本研究旨在对药用植物Kayu java (L. coromandelica (Houtt.))中分离的内生细菌进行鉴定和鉴定。稳定)。本研究是一项实验室实验研究。共筛选出8株菌株作为Kayu Jawa (L. coromandelica (Houtt))组织中具有代表性的细菌群落。稳定)。革兰氏染色显微镜观察。用Vitek 2进行生化鉴定。结果表明,芽孢杆菌是本研究的优势属,其次是葡萄球菌和泛菌属。根据所做的研究,从Kayu Jawa Lannea coromandelica (Houtt) Merr中分离到的内生细菌,有6株属于芽孢杆菌属,另外2株属于葡萄球菌属和Pantoea属。然而,为了探索这一有前景的应用,对宿主内生细菌的生物学和分布进行系统的研究是非常重要的。
Biochemical identification of endophytic bacteria isolates from medicinal plant kayu jawa lannea coromandelica (houtt.) merr
Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.