Ananias Pinto de Queiroz, Melissa Alexandra Romero, Gil Rodrigues dos Santos, Raimundo Wagner de Souza Aguiar
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引用次数: 0
摘要
瓜是巴西重要的农作物,受到包括蓟马在内的相关昆虫媒介的影响。本文研究了与Frankliniella sp.(蓟翅目:蓟科)相关的花生环斑病毒(GRSV)在巴西塞拉多(Cerrado)种植的西瓜中的存在情况。从Gurupi、Lagoa da Confusao、Formoso do Araguaia和Porto Nacional县(均属于巴西Tocantins州)的四个商业作物中收集了几种蓟马。从蓟马中获得的病毒总RNA被富集并用于进行RT-PCR。利用特异性引物,在所有昆虫样本中鉴定出一个644 bp的GRSV核衣壳片段,这使我们能够与其他地理区域描述的GRSV序列进行系统发育分析。研究结果表明,GRSV病毒在巴西塞拉多生物群的商品西瓜作物中广泛分布。与Frankliniella sp.的关联被强调为该高强度和先进农业地区最相关的媒介途径之一。此外,系统发育分析表明,来自南非和巴西的GRSV序列关系密切。这些发现可能有助于了解GRSV在巴西塞拉多生物群不同地区西瓜作物中的分布。
Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado
Citrullus lanatus (Thunb.) is an important crop in Brazil and affected by relevant insect vectors, including thrips (Thysanoptera: Thripidae). The present work was focused on evaluating the presence of Groundnut ringspot virus (GRSV) associated with Frankliniella sp. (Thysanoptera: Thripidae) in watermelon, C. lanatus , cultivated in the Brazilian Cerrado biome. Several species of thrips were collected from four commercial crops located in Gurupi, Lagoa da Confusao, Formoso do Araguaia and Porto Nacional counties, all of them belonging to the Brazilian state of Tocantins). The total viral RNA obtained from the thrips was enriched and used to perform ugh RT-PCR. Using specific primers, a 644 bp fragment of the GRSV nucleocapsid was identified in all insect samples, which allowed us to perform a phylogenetic analysis with GRSV sequences described for other geographical regions. The findings demonstrate the wide distribution of the GRSV virus in commercial watermelon crops in the Brazilian Cerrado Biome. The association with Frankliniella sp. is highlighted as one of the most relevant vector pathways in this region of high intensity and advanced agriculture. In addition, phylogenetic analysis demonstrated a close relationship between GRSV sequences from South Africa and Brazil. These findings may contribute to the understanding of GRSV distribution in watermelon crops in different localities of Brazilian Cerrado Biome.