薄片显微镜中系统、光学和计算自动化技术

Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP) Pub Date : 2019-06-24 DOI:10.1364/COSI.2019.CW4A.1

C. Müllenbroich, L. Silvestri, L. Turrini, A. P. D. Giovanna, I. Costantini, G. Mazzamuto, F. Vanzi, L. Sacconi, Carl J. Nelson, Jonathan M. Taylor, F. Pavone

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引用次数: 0

摘要

薄片显微镜在快速全器官获取方面表现出色，无论是在澄清的小鼠大脑还是本质上透明的斑马鱼幼虫中。在这里，我们介绍了显微镜自动化的技术和光学解决方案，包括自动聚焦，贝塞尔光束和前瞻性门控。

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Techniques for methodical, optical and computational automation in light-sheet microscopy

Light-sheet microscopy excels in fast whole-organ acquisitions either in clarified mouse brains or intrinsically transparent zebrafish larva. Here, we present our technical and optical solutions for microscope automation including autofocusing, Bessel beams and prospective gating.

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来源期刊

Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)

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