具有消毒剂特性的洗涤剂对体外细胞培养的潜在毒性评价

N. Dmytrukha, T. K. Korolenko, O. Lahutina, L. A. Lehkostup
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引用次数: 0

摘要

如今,具有消毒剂特性的洗涤剂被广泛应用于人类活动的各个领域,用于清洁表面、设备、工具,同时破坏传染性病原体。作为消毒剂的一种活性物质,它含有各种具有抗菌、抗病毒和抗真菌特性的化合物。为了确定这些物质的安全性,以及对动物进行毒理学研究,在替代的体外测试系统上进行了实验,特别是在细胞培养上,这可以快速评估和预测洗涤剂对人类和其他生物的潜在毒性。本研究的目的是在体外细胞培养实验中,通过确定洗涤剂和消毒剂的细胞毒性作用,评估它们对人类的潜在危险。材料和方法。采用细胞化学方法对具有消毒剂特性的清洁剂进行了研究:"Bioblysk"、"Blysk Plus"和"Blysk"(波兰PE "HYGIENICS"生产)对肺癌细胞(A-549系)的细胞毒作用进行了评估:甲基四氮唑蓝MTT试验和磺胺乙胺b CRB试验。结果表明,在天然溶液浓度为1.0%时,所有洗涤剂对a -549细胞的细胞毒作用最大。最低浓度0.03%和0.015%对细胞无毒性作用。根据所得结果进行probit分析,计算LC50值:biblysk - 0.15% (MTT试验)和0.15% (CRP试验);Blysk Plus LC50 - 0.25% (MTT试验)和0.2% (CRP试验);Blysk - 0.2% (MTT试验和CRP试验)。洗涤剂的LC50值比推荐的最低工作溶液浓度低10倍。结果表明,洗涤剂对A-549细胞的毒性作用主要表现在对线粒体功能和蛋白质合成的影响上。所研究药物的毒性作用的严重程度取决于其化学成分和培养液的pH、浓度。计算出的LC50值表明它们可能对呼吸系统有危险,因此需要适当的保护。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the potential toxicity of detergents with disinfectants properties on cell culture in vitro
Detergents with disinfectants properties today are widely used in various fields of human activity for cleaning surfaces, equipment, tools while destroying infectious agents. An active substance, as disinfectants, it contains various chemical compounds with antimicrobial, antiviral and antifungal properties. To establish the safety of these substances, along with toxicological studies on animals, experiments are conducted on alternative in vitro test systems, in particular on cell culture, which allows to quickly assess and predict the potential toxicity of detergents to humans and other living organisms. The purpouse of the study was assessment of the potential danger of detergents and disinfectants for humans by determining their cytotoxic effects in experiments on cell culture in vitro. Material and methods. Studies were performed to evaluate the cytotoxic effect of detergents with disinfectants properties : "Bioblysk", "Blysk Plus" and "Blysk" (manufactured by PE "HYGIENICS", Poland) on the lung cancer cells (line A-549) using cytochemical methods: MTT- with methyltetrazolium blue and CRB - test with sulforodamine B. Results. It was found that all detergents have the greatest cytotoxic effect on A-549 cells at a concentration of 1.0% of the native solution. The lowest concentrations 0.03% and 0.015% have no toxic effect on cells. With the help of probit analysis based on the obtained results, LC50 values were calculated: for Bioblysk - 0.15% (MTT test) and 0.15% (CRP test); for Blysk Plus LC50 - 0.25% (MTT test) and 0.2% (CRP test); for Blysk - 0.2% (MTT test and CRP test). LC50 values for detergents were 10 times lower than the recommended minimum concentrations of working solutions Conclusions. The obtained data indicate that the citotoxicity effect of detergents on A-549 cells was manifested by the effect on mitochondrial function and protein synthesis. The severity of studied drugs toxic effect depended on its chemical composition and pH, concentration in the incubation medium. The calculated LC50 values indicate their possible danger to the respiratory system, so they require appropriate protection.
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