体外无血清培养兔角膜上皮细胞。

Investigative ophthalmology Pub Date : 1976-08-01

M I Allman, R A Harper, M Yanoff, L J Curfman, J D Cameron, B A Flaxman

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引用次数: 0

摘要

正常兔角膜上皮原代培养物可以在无血清培养基中培养并繁殖6天。到第八天，大多数细胞已经停止分裂。无血清培养的上皮细胞显示出与添加血清培养的对照细胞相当的DNA合成活性。

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Rabbit corneal epithelial cells grown in vitro without serum.

Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.

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来源期刊

Investigative ophthalmology

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