M I Allman, R A Harper, M Yanoff, L J Curfman, J D Cameron, B A Flaxman
{"title":"体外无血清培养兔角膜上皮细胞。","authors":"M I Allman, R A Harper, M Yanoff, L J Curfman, J D Cameron, B A Flaxman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.</p>","PeriodicalId":14844,"journal":{"name":"Investigative ophthalmology","volume":"15 8","pages":"666-8"},"PeriodicalIF":0.0000,"publicationDate":"1976-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rabbit corneal epithelial cells grown in vitro without serum.\",\"authors\":\"M I Allman, R A Harper, M Yanoff, L J Curfman, J D Cameron, B A Flaxman\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.</p>\",\"PeriodicalId\":14844,\"journal\":{\"name\":\"Investigative ophthalmology\",\"volume\":\"15 8\",\"pages\":\"666-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1976-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Investigative ophthalmology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Investigative ophthalmology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Rabbit corneal epithelial cells grown in vitro without serum.
Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.