{"title":"细胞壁剪应力作用下细胞分裂后活性的变化","authors":"S. Hashimoto, Hiroki Yonezawa, Ryuya Ono","doi":"10.1115/imece2021-69689","DOIUrl":null,"url":null,"abstract":"\n Does cell orientation depend on the cell type in the shear stress field? Does that tendency change after the division? In this study, the behavior of each cell after division was tracked by time-lapse microscopic images through 24 hours of culture under a shear stress field. A constant shear stress field was applied to the cells in the Couette flow between the parallel walls: the lower static culture disc and the upper rotating disc. For comparison, four types of cells were used: C2C12 (mouse myoblast), HUVEC (human umbilical vein endothelial cells), 3T3-L1 (mouse adipose progenitor cells), and L929 (mouse fibroblast). The result is as follows. In the wall shear stress field of 1 Pa, HUVEC is oriented parallel to the flow, regardless of the division. In other cell types (C2C12, 3T3-L1, and L929) after division, the deformed cell tends to tilt to the direction parallel to the flow. The experimental results are expected to be applied to engineered tissue technologies.","PeriodicalId":314012,"journal":{"name":"Volume 5: Biomedical and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Cell Activity Change After Division Under Wall Shear Stress Field\",\"authors\":\"S. Hashimoto, Hiroki Yonezawa, Ryuya Ono\",\"doi\":\"10.1115/imece2021-69689\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Does cell orientation depend on the cell type in the shear stress field? Does that tendency change after the division? In this study, the behavior of each cell after division was tracked by time-lapse microscopic images through 24 hours of culture under a shear stress field. A constant shear stress field was applied to the cells in the Couette flow between the parallel walls: the lower static culture disc and the upper rotating disc. For comparison, four types of cells were used: C2C12 (mouse myoblast), HUVEC (human umbilical vein endothelial cells), 3T3-L1 (mouse adipose progenitor cells), and L929 (mouse fibroblast). The result is as follows. In the wall shear stress field of 1 Pa, HUVEC is oriented parallel to the flow, regardless of the division. In other cell types (C2C12, 3T3-L1, and L929) after division, the deformed cell tends to tilt to the direction parallel to the flow. The experimental results are expected to be applied to engineered tissue technologies.\",\"PeriodicalId\":314012,\"journal\":{\"name\":\"Volume 5: Biomedical and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Volume 5: Biomedical and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1115/imece2021-69689\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Volume 5: Biomedical and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1115/imece2021-69689","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cell Activity Change After Division Under Wall Shear Stress Field
Does cell orientation depend on the cell type in the shear stress field? Does that tendency change after the division? In this study, the behavior of each cell after division was tracked by time-lapse microscopic images through 24 hours of culture under a shear stress field. A constant shear stress field was applied to the cells in the Couette flow between the parallel walls: the lower static culture disc and the upper rotating disc. For comparison, four types of cells were used: C2C12 (mouse myoblast), HUVEC (human umbilical vein endothelial cells), 3T3-L1 (mouse adipose progenitor cells), and L929 (mouse fibroblast). The result is as follows. In the wall shear stress field of 1 Pa, HUVEC is oriented parallel to the flow, regardless of the division. In other cell types (C2C12, 3T3-L1, and L929) after division, the deformed cell tends to tilt to the direction parallel to the flow. The experimental results are expected to be applied to engineered tissue technologies.