甜菜体细胞胚的包封、萌发和转化

C. Tsai, J. Saunders
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引用次数: 10

摘要

甜菜(Beta vulgaris L.)生物技术无性系REL-2的体细胞胚是用absci - sic酸培养的愈伤组织获得的,用2%的海藻酸盐包被,随后发芽并转化为植株,这是最终常规生产人工种子所必需的初步努力。考察的因素包括胚大小、藻酸盐伴生液、冷藏时间和萌发基质。未包封的胚长类别(0.5-1.9 mm、2.0-2.9 mm或3.0 - 3.9 mm)不影响在无激素Murashige-Skoog (MS)培养基上的萌发率(98、99、100%)或成苗率(分别为87、89、87%)。海藻酸盐溶液(不含激素的MS培养基或h20)对发芽率(100%)没有影响,但在胚胎转化为植株方面存在差异(分别为81%和64%)。随后的实验考察了包被的胚胎在4°C下的冷藏时间,结果发现,经过21天的低温处理后,25°C下的发芽率与未保存的胚胎相比没有降低,但经过64天的低温处理后,在MS培养基中包被海藻酸盐的胚胎在25°C下的发芽率和转化率(分别为70%和45%)低于未保存的胚胎。褐藻酸盐水溶液在4℃条件下保存64 d,发芽率为60%
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Encapsulation, Germination, and Conversion of Somatic Embryos in Sugarbeet
Sugarbeet (Beta vulgaris L.) somatic embryos of biotech clone REL-2, obtained from callus grown with absci­ sic acid, were encapsulated with 2 % alginate and sub­ sequently germinated and converted into plantlets, in initial efforts necessary for eventual routine produc­ tion of artificial seeds. Factors examined were embryo size, alginate companion solution, cold storage dura­ tion, and germination substrate. Unencapsulated so­ matic embryo length category (0.5-1.9,2.0-2.9, or 3.0­ 3.9 mm) did not affect germination (98,99, 100%) or conversion (87, 89, 87%, respectively) rates into com­ plete plantlets on hormone-free Murashige-Skoog (MS) medium. Alginate companion solutions (either hor­ mone-free MS medium or H 20) had no differential ef­ fect on germination rate (100%) but did differ in con­ verting embryos to plantlets (81 vs. 64%, respectively). Subsequent experiments examining duration of cold storage of encapsulated embryos at 4°C found no lower rate of conversion at 25°C after 21 days of cold com­ pared with unstored embryos, but after 64 days of cold, the germination and conversion rates (70 and 45 %, respectively) at 25°C of embryos encapsulated with alginate in MS medium were lower compared with unstored embryos. With alginate in H20, germination after 64 days of storage at 4°C was 60%, and conver­
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