RAPD标记在木瓜性别鉴定中的应用

Comm. Horticulturae Journal Pub Date : 2019-10-04 DOI:10.29244/chj.1.1.1-5

R. Prihatini, Noflindawati, T. Budiyanti

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引用次数: 1

摘要

利用RAPD (Random Amplified Polymorphism DNA)标记对5个印尼番木瓜品种进行了分子性别鉴定。总的来说，14个引物中有12个在几个或所有被试品种上产生多态性条带。OPC04和RAPD2标记可用于确定所有品种的性别类型，而其他引物仅用于特定品种。唐开云谷品种可通过标记物进行区分:OPA11、OPA14、OPC14、RAPD2、RAPD3和RAPD5;本地sumi可通过以下标记确定:OPA01、OPA11、OPA14、OPC01、OPC04、RAPD2、RAPD3、RAPD5、RAPD6;使用OPC04、OPN09、RAPD2和RAPD5可以确定Merah Delima;可通过OPC01、OPC04、RAPD2、RAPD6测定湿度;而Sicincing Panjang可以通过OPA04、OPA11、OPA14、OPC04、RAPD2和RAPD3来确定。

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Application of RAPD Markers on Sex Determination of Papaya (Carica papaya)

Molecular sex determination of five varieties of Indonesian papaya were investigated using RAPD (Random Amplified Polymorphism DNA) markers. Overall, 12 of 14 primers produced polymorphic bands on either several or all tested varieties. The OPC04 and RAPD2 markers could be used determined sex types on all varieties, whereas others primers are only on certain varieties. The Tangkai Ungu variety can be differentiate by markers: OPA11, OPA14, OPC14, RAPD2, RAPD3, and RAPD5; the Lokal Sumani can be determine using markers: OPA01, OPA11, OPA14, OPC01, OPC04, RAPD2, RAPD3, RAPD5, and RAPD6; the Merah Delima could be determined using OPC04, OPN09, RAPD2, and RAPD5; the Dampit could be determined using OPC01, OPC04, RAPD2, and RAPD6; whereas the Sicincing Panjang could be determined using OPA04, OPA11, OPA14, OPC04, RAPD2, and RAPD3.

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Comm. Horticulturae Journal

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