3PC-038 Abstract withdrawn

Background In many hospital preparations in the form of syrups, dilution is often necessary, such as chloral syrup and chloral-containing bromide syrup diluted to 4.5% and 37%, respectively. But what is the degree of dilution that preserves the microbiological stability of the syrup? Purpose The aim of this work is to evaluate the anti-bacterial strength of various simple syrup forms after gradual dilutions with distilled water and artificial contamination of dilutions with a reference bacterium. Material and methods We prepared hot and cold sucrose syrup, fructose syrup and glucose syrup. The four syrups were then diluted at levels between 5% and 50% with distilled water artificially contaminated with Escherichia coli ATCC 25922 and then incubated at 37°C for 24 hours. The incubated dilutions were then seeded on CLED AGAR under sterile conditions. The inoculated boxes were also incubated at 37°C for 24 hours to 48 hours. A glucose assay by Architect c16000* in hot and cold sucrose solutions was conducted in the search for probable decomposition of sucrose into glucose and fructose. Results The appearance of the bacterial growth for the dilutions of the four solutions of glucose, cold sucrose, hot sucrose and fructose was respectively 9%, 15%, 35% and 45%. The glucose assay in the cold and hot sucrose solutions was 0.0 g/L and 0.11 g/L, respectively. Conclusion This simple study makes it possible to say that we can dilute a hospital preparation in the form of syrup for preparation needs at levels ranging from 9% to 45%, depending on the sugar and the method of preparation used. The fructose syrup is at the top for its antibacterial capacity followed by hot sucrose syrup and the cold one, leaving the glucose syrup last. Therefore, hospital preparations based on simple syrup of hot sucrose have more microbiological stability than those based on cold sucrose syrup. The appearance of fructose in hot sucrose syrup seems to be at the origin of its better microbiological stability. References and/or acknowledgements No conflict of interest.