安亭(Acalypha indica L.)的效力叶提取物对微乳杆菌幼虫和成虫的体外杀螨作用

Fitri Nurdiana, Rahmi Sugiharuti, B. S. Lukiswanto
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引用次数: 0

摘要

本研究旨在通过观察蚁叶提取物对微乳杆菌(Boophilus microplus)幼虫和成虫的死亡率,确定其体外杀螨效果。本研究设计为完全随机设计。本试验采用5种处理,分别为:Tween(80%)和aquadest (K-)、Neguvon (K+)、蚁叶提取物(浓度分别为3.125% (P1)、6.25% (P2)和12.5% (P3))。每次处理5次重复,每次重复使用6个微加贝幼虫期和5个微加贝成虫期。微加白刺幼虫期观察5 h,成虫期观察24 h。如果用针触碰微乳杆菌没有任何运动,就宣告其死亡。所得数据采用因子方差分析,并继续采用Duncan多重距离检验。结果表明,12.5%安亭叶提取物处理与Neguvon处理差异不显著。(p>0.05),但在成虫中与Neguvon差异显著(p<0.05)。统计检验结果表明,蚁叶提取物在体外具有杀螨作用。蚁叶提取物浓度越高,杀螨活性越高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Potency of Anting Anting (Acalypha indica L.) Leaf Extract as An Acaricide on Boophilus microplus in Larvae and Adult Stages In Vitro
The purpose of this study was to determine the potency of anting-anting leaf extract (Acalypha indica L.) as an acaricide against larval and adult Boophilus microplus in vitro by observing the mortality rate of B. microplus. The present study design was a completely randomized design. This study used five treatments, including: Tween 80 1% and aquadest (K-), Neguvon (K+), anting-anting leaf extract with a concentration of 3.125% (P1), 6.25% (P2), and 12.5% (P3 ). Each treatment used 5 repetitions and each repetition used 6 B. microplus larval stages and 5 B. microplus adults. Observations were made for 5 hours for the larval stage of B. microplus and 24 hours for the adult stage of B. microplus. Boophilus microplus is declared dead if there is no movement at all when touched with a needle. The data obtained were analyzed using factorial ANOVA and continued with Duncan's multiple distance test. The results showed that the 12.5% anting anting leaf extract treatment showed no significant difference with the treatment using Neguvon. (p>0.05) in larval stage B. microplus, but in adult B. microplus showed a significant difference with Neguvon (p<0.05). The results of the statistical test can be concluded that the extract of anting-anting leaf (A. indica L.) has the potential as an acaricide of B. microplus larval stage in vitro. The higher the concentration of anting anting leaf extract, the higher the acaricide activity.
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