{"title":"免疫组织化学在实验和临床中的重要作用","authors":"","doi":"10.2174/9789811439315120010008","DOIUrl":null,"url":null,"abstract":"Immunohistochemistry (IHC) is a well-known technique in the field of\nbiological and medical sciences. This technique is based on the principle of antigenantibody\ninteraction and is used for identification of cellular or tissue constituents, i.e.,\nan antigen by using a specific antibody. The binding of an antibody to an antigen is\nconfirmed either by labelled primary antibody itself or by using secondary labelling\nmethod such as fluorescence labelled antibody. Such interactions give information\nabout the cellular process occurring inside the cell. In last few years, huge amount of\ndata have been generated using IHC. Furthermore, adequate knowledge of this\ntechnique is required for the optimum result and its reproducibility. The detailed\ninformation about the tissue section, antigen retrieval (AR), increased sensitivity of the\ndetection systems and proper standardization are the key points for this technique. This\nprotocol will address overview of the technique, tissue preparation, microtome, antigen\nretrieval, antibodies and antigen fixation, detection methods, background reduction and\ntrouble shootings.","PeriodicalId":179247,"journal":{"name":"Protocols used in Molecular Biology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemistry as an Important Technique in Experimental and Clinical Practices\",\"authors\":\"\",\"doi\":\"10.2174/9789811439315120010008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Immunohistochemistry (IHC) is a well-known technique in the field of\\nbiological and medical sciences. This technique is based on the principle of antigenantibody\\ninteraction and is used for identification of cellular or tissue constituents, i.e.,\\nan antigen by using a specific antibody. The binding of an antibody to an antigen is\\nconfirmed either by labelled primary antibody itself or by using secondary labelling\\nmethod such as fluorescence labelled antibody. Such interactions give information\\nabout the cellular process occurring inside the cell. In last few years, huge amount of\\ndata have been generated using IHC. Furthermore, adequate knowledge of this\\ntechnique is required for the optimum result and its reproducibility. The detailed\\ninformation about the tissue section, antigen retrieval (AR), increased sensitivity of the\\ndetection systems and proper standardization are the key points for this technique. This\\nprotocol will address overview of the technique, tissue preparation, microtome, antigen\\nretrieval, antibodies and antigen fixation, detection methods, background reduction and\\ntrouble shootings.\",\"PeriodicalId\":179247,\"journal\":{\"name\":\"Protocols used in Molecular Biology\",\"volume\":\"15 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Protocols used in Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/9789811439315120010008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protocols used in Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/9789811439315120010008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Immunohistochemistry as an Important Technique in Experimental and Clinical Practices
Immunohistochemistry (IHC) is a well-known technique in the field of
biological and medical sciences. This technique is based on the principle of antigenantibody
interaction and is used for identification of cellular or tissue constituents, i.e.,
an antigen by using a specific antibody. The binding of an antibody to an antigen is
confirmed either by labelled primary antibody itself or by using secondary labelling
method such as fluorescence labelled antibody. Such interactions give information
about the cellular process occurring inside the cell. In last few years, huge amount of
data have been generated using IHC. Furthermore, adequate knowledge of this
technique is required for the optimum result and its reproducibility. The detailed
information about the tissue section, antigen retrieval (AR), increased sensitivity of the
detection systems and proper standardization are the key points for this technique. This
protocol will address overview of the technique, tissue preparation, microtome, antigen
retrieval, antibodies and antigen fixation, detection methods, background reduction and
trouble shootings.