免疫组织化学在实验和临床中的重要作用

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引用次数: 0

摘要

免疫组织化学(IHC)是生物和医学领域的一项著名技术。该技术基于抗原-抗体相互作用原理,用于细胞或组织成分的鉴定,即通过使用特异性抗体鉴定抗原。抗体与抗原的结合可以通过标记的一级抗体本身或使用二级标记方法(如荧光标记抗体)来确认。这种相互作用提供了细胞内发生的细胞过程的信息。在过去的几年里,使用IHC产生了大量的数据。此外,为了获得最佳结果及其可重复性,需要充分了解该技术。详细的组织切片信息、抗原检索(AR)、提高检测系统的灵敏度和适当的标准化是该技术的关键。本协议将涉及技术概述,组织准备,切片机,抗原检索,抗体和抗原固定,检测方法,背景还原和故障排除。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemistry as an Important Technique in Experimental and Clinical Practices
Immunohistochemistry (IHC) is a well-known technique in the field of biological and medical sciences. This technique is based on the principle of antigenantibody interaction and is used for identification of cellular or tissue constituents, i.e., an antigen by using a specific antibody. The binding of an antibody to an antigen is confirmed either by labelled primary antibody itself or by using secondary labelling method such as fluorescence labelled antibody. Such interactions give information about the cellular process occurring inside the cell. In last few years, huge amount of data have been generated using IHC. Furthermore, adequate knowledge of this technique is required for the optimum result and its reproducibility. The detailed information about the tissue section, antigen retrieval (AR), increased sensitivity of the detection systems and proper standardization are the key points for this technique. This protocol will address overview of the technique, tissue preparation, microtome, antigen retrieval, antibodies and antigen fixation, detection methods, background reduction and trouble shootings.
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