啤酒和葡萄酒中赭曲霉毒素A快速检测的无阻标免疫传感器

2020 IEEE International Workshop on Metrology for Agriculture and Forestry (MetroAgriFor) Pub Date : 2020-11-04 DOI:10.1109/MetroAgriFor50201.2020.9277594

Francesca Malvano, D. Albanese, R. Pilloton

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引用次数: 1

摘要

本文报道了一种检测食品中赭曲霉毒素A的无阻抗标记免疫传感器的研制。研究并比较了用于OTA检测的单克隆抗体的两种不同固定方案，目的是开发一种能够检测相当于欧洲食品立法规定的最高水平的OTA量的分析装置。活性二茂铁作为电子传递介质，提高了免疫传感器的电学性能，检测限为0.25 ng/mL。这种免疫传感器被用来分析葡萄酒和啤酒样品中已知的OTA含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Impedimetric label - free immunosensor for rapid detection of Ochratoxin A in beer and wine

In this work, the development of an impedimetric label-free immunosensor for the detection of Ochratoxin A in food products is reported. Two different immobilization schemes of a monoclonal antibody for OTA detection were investigated and compared, with the aim to develop an analytical device able to detect an OTA amount equal to the maximum levels imposed by European legislation for food products. activated ferrocene as electron - transferring mediator, improved the electrical properties of the immunosensor, which showed a limit of detection equal to 0.25 ng/mL. This immunosensor was used to analyze wine and beer samples spiked with known OTA amounts..

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来源期刊

2020 IEEE International Workshop on Metrology for Agriculture and Forestry (MetroAgriFor)

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