转基因小鼠厚肝组织培养的三维微结构集成生物反应器系统

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2012-03-05 DOI:10.1109/NEMS.2012.6196746

S. Sivashankar, S. Puttaswamy, Hui-Ling Lin, Shih-Mo Yang, Hung-Po Chen, C. Yeh, Cheng-Hsien Liu

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引用次数: 2

摘要

本文提出了一种具有三维聚乙二醇二丙烯酸酯(PEGDA)结构的培养孔生物反应器体系，以提供三维培养条件。培养基通过每个生物反应器出口的喷嘴状流道扩散，组织被保持在PEGDA微结构之间。这有助于将所需的营养物质输送到组织的核心，消除了传质问题。基于PEGDA的微结构的存在导致肝组织和间皮细胞周围的介质更好地循环，提供三维微环境并保持完整的结构。流速为20 μl/min，每12h改变一次流速方向，可有效去除废物和毒素。2mm厚的组织切片培养12天，活细胞76%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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3D microstructure integrated bioreactor system for transgenic mice thick liver tissue culture

A bioreactor system with 3-diementional (3D) Poly ethylene glycol diacrylate (PEGDA) structures in the culture-well to provide 3D culturing conditions is proposed here. The culture medium diffused through the tissue cause of the nozzle shaped flow channel at the outlet of each bioreactor and tissue being held between PEGDA microstructures. This helps in delivering the required nutrients to core of the tissue, eliminating the mass transfer problem. Presence of PEGDA based microstructures resulted in better circulation of medium around the liver tissue and mesothelial cells providing 3D microenvironment and to retain intact architecture. The flow rate was 20 μl/min and the flow direction is changed every 12hrs for effective removal of waste products and toxins. Tissues slices of 2mm thick have been cultured for 12 days with 76% viable cells.

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来源期刊

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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