脂肪酸在离体灌注大鼠肾脏中的转运和代谢。

M E Trimble, W W Harrington, R H Bowman
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引用次数: 0

摘要

用离体大鼠肾脏制剂研究了内源性脂肪酸的转运和代谢。结果倾向于证实丙戊酸对肾脏脂肪酸摄取的抑制作用,但在使用的浓度下,丙戊酸对改变组织脂质中棕榈酸14C的分布无效。α -溴铝酸酯(α bp) (0.4 mM),一种脂肪酸氧化抑制剂,引起14C棕榈酸盐摄取的初始增强,组织磷脂的酯化反应减少,14C作为游离脂肪酸在组织中恢复增加。这种增加对脂肪酸的进入没有抑制作用。α BP对14C辛酸盐的摄取没有影响,似乎不影响一般代谢。结果与α bp与肾脂肪酸结合蛋白相互作用的可能性一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fatty acid transport and metabolism in the isolated perfused rat kidney.

Transport and metabolism of endogenous fatty acids was studied using an isolated rat kidney preparation. Results tended to confirm the inhibitory effects of probenecid on fatty acid uptake by the kidney but, at the concentration used, probenecid was ineffective in changing the distribution of 14C palmitate within tissue lipids. Alpha-bromopalmitate (alphaBP) (0.4 mM), an inhibitor of fatty acid oxidation, caused an initial enhancement of 14C palmitate uptake, a decrease in esterification into tissue phospholipids and an increase in 14C recovered as free fatty acid in the tissue. This increase had no inhibitory effect on entry of fatty acids. alpha BP had no effect on 14C octanoate uptake and appeared not to affect general metabolism. The results are consistent with the possibility that alphaBP interacts with a renal fatty acid binding protein.

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