测量铽与DNA的结合

Gene Yonuschot, Gerry Robey, George W. Mushrush , Donald Helman , George Vande Woude
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引用次数: 12

摘要

这进一步证明了铽(III)可以用作DNA探针。用两种新方法测定了铽与DNA结合的化学计量学。在第一种方法中,用放射性铽-160滴定小牛胸腺DNA,这是常见的铽-159的同位素。得到的dna -铽络合物被捕获并在微孔过滤器上测量。在第二种方法中,在219 nm处发现了铽的紫外吸收峰,并用于测量化学计量。通过这两种方法,DNA中每三个可用磷酸基团的结合化学计量为1 Tb(III)。最后,建立了一种利用铽-160快速测定溶液中核酸含量的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Measurement of binding of terbium to DNA

This communication demonstrates further that terbium(III) can be used as a probe for DNA. The stoichiometry of terbium binding to DNA was measured by two new methods. In the first method, calf-thymus DNA was titrated with radioactive terbium-160, which is an isotope of the common terbium-159. The resulting DNA-terbium complex was trapped and measured on millipore filters. In the second method, a peak of UV absorption of terbium was found at 219 nm and was used to measure stoichiometry. By both methods, the stoichiometry of binding was one Tb(III) for each three available phosphate groups in DNA. Finally, a rapid method was developed using terbium-160 to measure the amount of nucleic acid in a solution.

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