{"title":"尿游离皮质醇的放射免疫测定。","authors":"R L Sokoloff, R L Hilderbrand","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran-coated charcoal to separate bound and unbound cortisol. 3H-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intraassay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 2","pages":"133-9"},"PeriodicalIF":0.0000,"publicationDate":"1977-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Radioimmunoassay of urinary free cortisol.\",\"authors\":\"R L Sokoloff, R L Hilderbrand\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran-coated charcoal to separate bound and unbound cortisol. 3H-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intraassay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week.</p>\",\"PeriodicalId\":12869,\"journal\":{\"name\":\"Health laboratory science\",\"volume\":\"14 2\",\"pages\":\"133-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1977-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Health laboratory science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Health laboratory science","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran-coated charcoal to separate bound and unbound cortisol. 3H-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intraassay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week.
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