支持脂质双层微阵列的表面和内部的微流体通道

P. Kim, Sang Eun Lee, Ho-Sup Jung, Hea-Yeon Lee, T. Kawai, H. Jeong, K. Suh
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引用次数: 2

摘要

我们提出了一种简单的软光刻方法,用于在微流体通道表面和内部绘制支持脂质双分子层(SLB)膜。采用微接触印刷或毛细模塑技术在玻璃基板上制备聚乙二醇基聚合物的微图案。与玻璃对照相比,图形化的PEG表面在二维表面上的脂质吸附减少了97plusmn0.5%,在微流控通道内的脂质吸附减少了95plusmn1.2%。原子力显微镜测量表明,脂质囊泡的沉积导致与暴露的底物亲水性相互作用的囊泡融合形成SLB膜。此外,通过测量生物素(配体)标记的脂质双分子层与链亲和素(受体)之间的结合相互作用,测试了图案slb的功能。在平面衬底上制备了空间分辨率为~500 nm的SLB阵列,在微流控通道内制备了空间分辨率为~1 nm的SLB阵列
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Supported lipid bilayers microarrays onto a surface and inside microfluidic channels
We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary molding. The patterned PEG surfaces have shown 97plusmn0.5% reduction in lipid adsorption onto two dimensional surfaces and 95plusmn1.2% reduction inside microfluidic channels in comparison to glass control. Atomic force microscopy measurements indicated that the deposition of lipid vesicles led to the formation of SLB membranes by vesicle fusion due to hydrophilic interactions with the exposed substrate. Furthermore, the functionality of the patterned SLBs was tested by measuring the binding interactions between biotin (ligand)-labeled lipid bilayer and streptavidin (receptor). SLB arrays were fabricated with spatial resolution down to ~500 nm on flat substrate and ~1 mum inside microfluidic channels, respectively
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