cry1ba1介导转基因伯格氏菌和柑橘对亚洲柑橘木虱的毒性研究

V. Orbović, Seyed Ali Ravanfar, D. Achor, T. Shilts, Freddy Ibanez-Carrasco, R. Banerjee, Choaa El-Mohtar, L. Stelinski, B. Bonning
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引用次数: 2

摘要

柑桔木虱(Diaphorina citri)是柑桔绿色病的病原菌,在全球范围内严重影响柑桔生产。由于目前重复使用化学杀虫剂对这种昆虫的管理和随后的森林保护是不可持续的,我们研究了细菌衍生的杀虫蛋白Cry1Ba1通过转基因柑橘植物传递的潜在用途。在将印度咖喱叶树Bergera koenigii转化为Cry1Ba1表达的诱捕植物后,我们生产了Duncan葡萄柚、Citrus paridisi、Valencia甜橙、Citrus sinensis和Carrizo citrange、C. sinensis x Poncirus trifoliata等表达Cry1Ba1的转基因植物。证实了cry1ba1基因的存在和cry1ba1的转录。Western blot检测Cry1Ba1在大多数病例中得到证实。与野生型植物相比,表达Cry1Ba1的转基因Duncan和Valencia的叶盘表现出“延迟衰老”的表型,与转基因B. koenigii的观察结果相似。在生物试验中,表达Cry1Ba1的转基因科尼氏木虱和瓦伦西亚甜橙植株的成虫存活率显著降低,而转基因邓肯葡萄柚和卡里佐柑橘的成虫存活率无显著降低。与取食野生型植物的木虱相比,取食转基因植物的木虱肠道上皮受到损伤,与Cry1Ba1的作用方式一致。这些结果表明,一种细菌杀虫蛋白在科尼氏白杆菌和瓦伦西亚甜橙中的转基因表达,为柑橘枯萎病的防治提供了一种可行的选择,可能有助于解决柑橘绿化病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cry1Ba1-mediated toxicity of transgenic Bergera koenigii and Citrus sinensis to the Asian citrus psyllid Diaphorina citri
The Asian citrus psyllid, Diaphorina citri, vectors the bacterial causative agent of citrus greening disease, which has severely impacted citrus production on a global scale. As the current repeated application of chemical insecticides is unsustainable for management of this insect and subsequent protection of groves, we investigated the potential use of the bacteria-derived pesticidal protein, Cry1Ba1, when delivered via transgenic citrus plants. Having demonstrated transformation of the Indian curry leaf tree, Bergera koenigii, for Cry1Ba1 expression for use as a trap plant, we produced transgenic plants of Duncan grapefruit, Citrus paridisi, Valencia sweet orange, Citrus sinensis, and Carrizo citrange, C. sinensis x Poncirus trifoliata, for expression of Cry1Ba1. The presence of the cry1ba1 gene, and cry1ba1 transcription were confirmed. Western blot detection of Cry1Ba1 was confirmed in most cases. When compared to those from wild-type plants, leaf discs from transgenic Duncan and Valencia expressing Cry1Ba1 exhibited a “delayed senescence” phenotype, similar to observations made for transgenic B. koenigii. In bioassays, significant reductions in the survival of adult psyllids were noted on transgenic B. koenigii and Valencia sweet orange plants expressing Cry1Ba1, but not on transgenic Duncan grapefruit or Carrizo citrange. In contrast to psyllids fed on wild type plants, the gut epithelium of psyllids fed on transgenic plants was damaged, consistent with the mode of action of Cry1Ba1. These results indicate that the transgenic expression of a bacterial pesticidal protein in B. koenigii and Valencia sweet orange offers a viable option for management of D. citri, that may contribute to solutions that counter citrus greening disease.
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