{"title":"FLIM-FRET数据的无监督分析","authors":"F. Masia, P. Borri, W. Dewitte, W. Langbein","doi":"10.1109/IPC53466.2022.9975544","DOIUrl":null,"url":null,"abstract":"We have developed an unsupervised method to analyze fluorescence lifetime imaging microscopy data based on non-negative matrix factorization. Additionally, by modelling the Förster resonant energy transfer process between donor and acceptor fluorophores, we retrieve the dynamics and the distribution of donor-acceptor pairs.","PeriodicalId":202839,"journal":{"name":"2022 IEEE Photonics Conference (IPC)","volume":"45 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unsupervised analysis of FLIM-FRET data\",\"authors\":\"F. Masia, P. Borri, W. Dewitte, W. Langbein\",\"doi\":\"10.1109/IPC53466.2022.9975544\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have developed an unsupervised method to analyze fluorescence lifetime imaging microscopy data based on non-negative matrix factorization. Additionally, by modelling the Förster resonant energy transfer process between donor and acceptor fluorophores, we retrieve the dynamics and the distribution of donor-acceptor pairs.\",\"PeriodicalId\":202839,\"journal\":{\"name\":\"2022 IEEE Photonics Conference (IPC)\",\"volume\":\"45 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2022 IEEE Photonics Conference (IPC)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/IPC53466.2022.9975544\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2022 IEEE Photonics Conference (IPC)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/IPC53466.2022.9975544","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
We have developed an unsupervised method to analyze fluorescence lifetime imaging microscopy data based on non-negative matrix factorization. Additionally, by modelling the Förster resonant energy transfer process between donor and acceptor fluorophores, we retrieve the dynamics and the distribution of donor-acceptor pairs.
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